Ischemia/reperfusion induces the recruitment of leukocytes from whole blood under flow conditions

被引:45
作者
Cassie, S
Masterson, MF
Polukoshko, A
Viskovic, MM
Tibbles, LA
机构
[1] Univ Calgary, Dept Med, Immunol Res Grp, Calgary, AB, Canada
[2] Univ Calgary, Dept Physiol, Immunol Res Grp, Calgary, AB, Canada
关键词
adhesion molecule; anoxia; eosinophil; hypoxia; intercellular adhesion molecule 1; lymphocyte; monocyte; neutrophil; peripheral blood mononuclear cell; polymorphonuclear cell; selectin; vascular cell adhesion molecule 1; free radicals;
D O I
10.1016/j.freeradbiomed.2004.02.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ischemia/reperfusion (I/R) occurs in a number of pathological conditions, including myocardial infarction, stroke, and organ transplantation. During the reperfusion phase, leukocytes are recruited into affected tissues, where they can cause tissue damage and organ failure. Various in vitro models have been developed to study the role of adhesion molecules in I/R-mediated leukocyte recruitment. These models traditionally use isolated leukocytes and static conditions and, therefore, may not recapitulate the in vivo situation. We developed two novel in vitro models of I/R-mediated leukocyte recruitment in which leukocyte recruitment was examined using whole blood under shear conditions. Chemical treatments were used to mimic I/R in the first model, while sequential exposure to hypoxia/reoxygenation (H/R) was used to mimic I/R in the second model. We found that leukocytes were recruited from whole blood under shear conditions to endothelial cells treated with chemically induced I/R or H/R. In both models, mRNA for intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin was upregulated. The role of adhesion molecules in leukocyte recruitment differed slightly between the two models, with E-selectin and VCAM-1 playing approximately equal roles in leukocyte recruitment in the chemically induced I/R model and VCAM-1 being a central mediator of leukocyte recruitment in the H/R model. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:1102 / 1111
页数:10
相关论文
共 33 条
  • [1] BILLUPS KL, 1995, J LAB CLIN MED, V125, P626
  • [2] Intact mitochondrial electron transport function is essential for signalling by hydrogen peroxide in cardiac myocytes
    Bogoyevitch, MA
    Ng, DCH
    Court, NW
    Draper, KA
    Dhillon, A
    Abas, L
    [J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2000, 32 (08) : 1469 - 1480
  • [3] Hydrogen peroxide dose dependent induction of cell death or hypertrophy in cardiomyocytes
    Chen, QM
    Tu, VC
    Wu, YW
    Bahl, JJ
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2000, 373 (01) : 242 - 248
  • [4] Cerebral protection in homozygous null ICAM-1 mice after middle cerebral artery occlusion - Role of neutrophil adhesion in the pathogenesis of stroke
    Connolly, ES
    Winfree, CJ
    Springer, TA
    Naka, Y
    Liao, H
    Yan, SD
    Stern, DM
    Solomon, RA
    GutierrezRamos, JC
    Pinsky, DJ
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (01) : 209 - 216
  • [5] C5 is required for CD49d expression on neutrophils and VCAM expression on vascular endothelial cells following mesenteric ischemia/reperfusion
    Fleming, SD
    Anderson, J
    Wilson, F
    Shea-Donohue, T
    Tsokos, GC
    [J]. CLINICAL IMMUNOLOGY, 2003, 106 (01) : 55 - 64
  • [6] Severe inflammatory defect and reduced viability in CD18 and E-selectin double-mutant mice
    Forlow, SB
    White, EJ
    Barlow, SC
    Feldman, SH
    Lu, H
    Bagby, GJ
    Beaudet, AL
    Bullard, DC
    Ley, K
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2000, 106 (12) : 1457 - 1466
  • [7] POLYMORPHONUCLEAR LEUKOCYTE ACCUMULATION IN BRAIN-REGIONS WITH LOW BLOOD-FLOW DURING THE EARLY POSTISCHEMIC PERIOD
    HALLENBECK, JM
    DUTKA, AJ
    TANISHIMA, T
    KOCHANEK, PM
    KUMAROO, KK
    THOMPSON, CB
    OBRENOVITCH, TP
    CONTRERAS, TJ
    [J]. STROKE, 1986, 17 (02) : 246 - 253
  • [8] Ichikawa H, 1997, CIRC RES, V81, P922
  • [9] H2O2-mediated permeability:: role of MAPK and occludin
    Kevil, CG
    Oshima, T
    Alexander, B
    Coe, LL
    Alexander, JS
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2000, 279 (01): : C21 - C30
  • [10] Endothelial cells exposed to anoxia/reoxygenation are hyperadhesive to T-lymphocytes: Kinetics and molecular mechanisms
    Kokura, S
    Wolf, RE
    Yoshikawa, T
    Ichikawa, H
    Granger, DN
    Aw, TY
    [J]. MICROCIRCULATION, 2000, 7 (01) : 13 - 23