A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum

被引：34

作者：

Dixon, Matthew W. A. ^{[1
]}

Peatey, Christopher L. ^{[1
]}

Gardiner, Donald L. ^{[1
]}

Trenholme, Katharine R. ^{[1
]}

机构：

[1] Queensland Inst Med Res, Malaria Biol Lab, Herston, Qld 4006, Australia

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2009年 / 163卷 / 02期

基金：

英国医学研究理事会;

关键词：

Plasmodium falciparum; Malaria; Gametocyte; CONTINUOUS CULTURE; MALARIA PARASITES; STAGE; COMMITMENT; EXPRESSION; SEX;

D O I：

10.1016/j.molbiopara.2008.10.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Study of the formation of the sexual blood stages of the malaria parasite has been significantly hampered by the absence of a reliable, reproducible assay devoid of operator bias and error. Here we report on the development of an assay utilizing a green fluorescent protein chimera of the early sexual blood stage protein Pfs16 as a marker for commitment to gametocytogenesis. Analysis of parasites via fluorescence activated cell sorting allows for the accurate assessment of gametocyte production well before morphological changes are apparent. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：123 / 126

页数：4

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