Scaffold electrodes based on thioctic acid-capped gold nanoparticles coordinated Alcohol Dehydrogenase and Azure A films for high performance biosensor

被引：13

作者：

Gomez-Anquela, C. ^{[1
]}

Garcia-Mendiola, T. ^{[1
]}

Abad, Jose M. ^{[1
]}

Pita, M. ^{[3
]}

Pariente, F. ^{[1
]}

Lorenzo, E. ^{[1
,2
]}

机构：

[1] Univ Autonoma Madrid, Dept Analyt Chem, E-28049 Madrid, Spain

[2] IMDEA Nanociencia, Madrid, Spain

[3] CSIC, Inst Catalisis & Petr Quim, Madrid 28049, Spain

来源：

BIOELECTROCHEMISTRY | 2015年 / 106卷

关键词：

Alcohol dehydrogenase; Biosensor; Gold nanoparticles; Electrografting; AMPEROMETRIC BIOSENSOR; GAS-CHROMATOGRAPHY; CARBON NANOTUBES; HYBRID SYSTEMS; NADH OXIDATION; ETHANOL; PROTEIN; ELECTROCATALYSIS; IMMOBILIZATION; PHENOTHIAZINE;

D O I：

10.1016/j.bioelechem.2015.06.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanometric size gold nanoparticles capped with thiotic add are used to coordinate with the Zn (II) present in the catalytic center of Alcohol Dehydrogenase (ADH). In combination with the NADH oxidation molecular catalyst Azure A, electrografted onto carbon screen-printed electrodes, they are used as scaffold electrodes for the construction of a very efficient ethanol biosensor. The final biosensing device exhibits a highly efficient ethanol oxidation with low overpotential of -0.25 V besides a very good analytical performance with a detection limit of 0.14 +/- 0.01 mu M and a stable response for more than one month. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：335 / 342

页数：8

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