Erythrocyte copper chaperone for superoxide dismutase and superoxide dismutase as biomarkers for hepatic copper concentrations in Labrador retrievers

被引:7
作者
Dirksen, K. [1 ]
Roelen, Y. S. [1 ]
van Wolferen, M. E. [1 ]
Kruitwagen, H. S. [1 ]
Penning, L. C. [1 ]
Burgener, I. A. [1 ]
Spee, B. [1 ]
Fieten, H. [1 ]
机构
[1] Univ Utrecht, Fac Vet Med, Dept Clin Sci Compan Anim, Yalelaan 108, NL-3584 CM Utrecht, Netherlands
关键词
Biomarker; Copper; Copper chaperone for superoxide Cu/Zn dismutase 1; Hepatitis; Superoxide dismutase 1; MESSENGER-RNA; DEFICIENT RATS; IN-VIVO; LIVER; TOXICOSIS; TERRIERS; PROTEIN; GENE; ZINC; ACCUMULATION;
D O I
10.1016/j.tvjl.2016.10.007
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Hereditary hepatic copper accumulation in Labrador retrievers leads to hepatitis with fibrosis and eventually cirrhosis. The development of a non-invasive blood-based biomarker for copper status in dogs could be helpful in identifying dogs at risk and to monitor copper concentrations during treatment. In this study, two cellular copper metabolism proteins, Cu/Zn superoxide dismutase (SOD1) and its chaperone (copper chaperone for SOD1, CCS) were measured in erythrocytes and tested for association with hepatic copper concentrations in 15 Labrador retrievers with normal or increased hepatic copper concentrations. Antibodies against CCS and SOD1 were applicable for use in canine specimens. This was demonstrated by the loss of immune-reactive bands for CCS and SOD1 in siRNA treated canine bile duct epithelial cells. Erythrocyte CCS and CCS/SOD1 ratios were decreased 2.37 (P < 0.001) and 3.29 (P < 0.001) fold in the high copper group compared to the normal copper group. Erythrocyte CCS and CCS/SOD1 ratio are potential new biomarkers for hepatic copper concentrations in Labrador retrievers and could facilitate early diagnosis and treatment monitoring for copper-associated hepatitis in dogs. (C) 2016 Elsevier Ltd. All rights reserved.
引用
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页码:1 / 6
页数:6
相关论文
共 47 条
[1]  
[Anonymous], NAT GENET
[2]   CCS mRNA transcripts and serum CCS protein as copper marker in adults suffering inflammatory processes [J].
Araya, Magdalena ;
Gutierrez, Ricardo ;
Arredondo, Miguel .
BIOMETALS, 2014, 27 (04) :645-652
[3]   Copper modulates the degradation of copper chaperone for Cu,Zn superoxide dismutase by the 26 S proteosome [J].
Bertinato, J ;
L'Abbé, MR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (37) :35071-35078
[4]   Copper deficiency induces the upregulation of the copper chaperone for Cu/Zn superoxide dismutase in weanling male rats [J].
Bertinato, J ;
Iskandar, M ;
L'Abbé, MR .
JOURNAL OF NUTRITION, 2003, 133 (01) :28-31
[5]   Decreased Erythrocyte CCS Content is a Biomarker of Copper Overload in Rats [J].
Bertinato, Jesse ;
Sherrard, Lindsey ;
Plouffe, Louise J. .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2010, 11 (07) :2624-2635
[6]   Neutron activation analysis and X-ray Rayleigh and Raman scattering of hair and nail clippings as noninvasive bioindicators for Cu liver status in Labrador Retrievers [J].
Bode, Peter ;
Maretti Silveira Bueno, Maria Izabel ;
Bortoleto, Gisele G. ;
Hoffmann, Gaby ;
van den Ingh, Ted S. G. A. M. ;
Rothuizen, Jan .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 390 (06) :1653-1658
[7]   Differential impact of copper deficiency in rats on blood cuproproteins [J].
Broderius, Margaret A. ;
Prohaska, Joseph R. .
NUTRITION RESEARCH, 2009, 29 (07) :494-502
[8]   MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments [J].
Bustin, Stephen A. ;
Beaulieu, Jean-Francois ;
Huggett, Jim ;
Jaggi, Rolf ;
Kibenge, Frederick S. B. ;
Olsvik, Pal A. ;
Penning, Louis C. ;
Toegel, Stefan .
BMC MOLECULAR BIOLOGY, 2010, 11
[9]   The copper chaperone CCS directly interacts with copper/zinc superoxide dismutase [J].
Casareno, RLB ;
Waggoner, D ;
Gitlin, JD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (37) :23625-23628
[10]   Hepatitis and increased copper levels in a Dalmatian [J].
Cooper, VL ;
Carlson, MP ;
Jacobson, J ;
Schneider, NR .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1997, 9 (02) :201-203