Expression of bovine Mx1 protein inhibits the replication of foot-and-mouth disease virus in BHK-21 cells

被引：11

作者：

Cai, K. J. ^{[1
,3
]}

Meng, Q. L. ^{[1
]}

Qiao, J. ^{[1
]}

Huang, J. ^{[2
]}

Zhang, Z. C. ^{[1
]}

Wang, G. C. ^{[1
]}

Wang, J. W. ^{[1
]}

Chen, C. F. ^{[1
]}

机构：

[1] Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Peoples R China

[2] Acad Xinjiang, Res Inst Vet Anim Sci, Urumqi 830000, Peoples R China

[3] Control & Diag Ctr Anim Dis Urumqi City, Urumqi, Xinjiang, Peoples R China

来源：

ACTA VIROLOGICA | 2013年 / 57卷 / 04期

基金：

中国国家自然科学基金;

关键词：

foot-and-mouth disease virus; Mx1; protein; BHK-21; cells; VESICULAR STOMATITIS-VIRUS; ALPHA/BETA INTERFERON; FUNCTIONAL DIVERSITY; IDENTIFICATION; RESISTANCE; GENE; OLIGOMERS; GTPASE; MICE; UK;

D O I：

10.4149/av_2013_04_429

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mx proteins belonging to the dynamin superfamily of large GTPases inhibit replication of a wide range of RNA viruses. In this study, we examined whether bovine Mx1 protein could interfere with the replication of foot-and-mouth disease virus (FMDV). For this purpose we established cloned BHK-21 cells expressing bovine Mx1 protein (BM1 cells) and infected them with FMDV serotype O. Cloned BHK-21 cells expressing neomycin resistance instead of Mx1 protein (BH1 cells) and original BHK-21 cells served as negative controls. The results showed that the expression of bovine Mx1 protein reduced viral yields by 90% and levels of viral VP1 mRNA by 60%. These findings correlated with a significant reduction of viral antigen detectable in infected cells by immunofluorescent assay. These results demonstrate that bovine Mx1 protein interferes with the replication of FMDV.

引用

页码：429 / 434

页数：6

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