Liquid Biopsy: Is There an Advantage to Analyzing Circulating Exosomal DNA Compared to cfDNA or Are They the Same?

被引：47
作者：
Kahlert, Christoph [1 ]
机构：
[1] Tech Univ Dresden, Univ Hosp, Dept Visceral Thorac & Vasc Surg, Dresden, Germany
来源：
CANCER RESEARCH | 2019年 / 79卷 / 10期
关键词：
STRANDED GENOMIC DNA; CANCER; KRAS;
D O I：
10.1158/0008-5472.CAN-19-0019
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Cancer is one of the leading causes of death worldwide. This life-threatening disease requires novel strategies for the early detection and therapy response prediction. Circulating DNA was first described 70 years ago. However, only the recent evolution in the PCR-based sequencing techniques allow the minimally invasive molecular profiling of circulating mutant DNA from small-volume "liquid biopsies" such as blood, urine, or saliva. In this article, we aim to summarize the fast-growing evidence for cfDNA and exosomal DNA as minimally invasive diagnostic markers in solid tumors and to highlight their opposing diagnostic advantages and disadvantages.
引用
收藏
页码：2462 / 2465
页数：4
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  • [1] High prevalence of mutant KRAS in circulating exosome-derived DNA from early-stage pancreatic cancer patients
    Allenson, K.
    Castillo, J.
    San Lucas, F. A.
    Scelo, G.
    Kim, D. U.
    Bernard, V.
    Davis, G.
    Kumar, T.
    Katz, M.
    Overman, M. J.
    Foretova, L.
    Fabianova, E.
    Holcatova, I.
    Janout, V.
    Meric-Bernstam, F.
    Gascoyne, P.
    Wistuba, I.
    Varadhachary, G.
    Brennan, P.
    Hanash, S.
    Li, D.
    Maitra, A.
    Alvarez, H.
    [J]. ANNALS OF ONCOLOGY, 2017, 28 (04) : 741 - 747
  • [2] Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences
    Balaj, Leonora
    Lessard, Ryan
    Dai, Lixin
    Cho, Yoon-Jae
    Pomeroy, Scott L.
    Breakefield, Xandra O.
    Skog, Johan
    [J]. NATURE COMMUNICATIONS, 2011, 2
  • [3] Circulating Nucleic Acids Are Associated With Outcomes of Patients With Pancreatic Cancer
    Bernard, Vincent
    Kim, Dong U.
    San Lucas, F. Anthony
    Castillo, Jonathan
    Allenson, Kelvin
    Mulu, Feven C.
    Stephens, Bret M.
    Huang, Jonathan
    Semaan, Alexander
    Guerrero, Paola A.
    Kamyabi, Nabiollah
    Zhao, Jun
    Hurd, Mark W.
    Koay, Eugene J.
    Taniguchi, Cullen M.
    Herman, Joseph M.
    Javle, Milind
    Wolff, Robert
    Katz, Matthew
    Varadhachary, Gauri
    Maitra, Anirban
    Alvarez, Hector A.
    [J]. GASTROENTEROLOGY, 2019, 156 (01) : 108 - +
  • [4] Detection and localization of surgically resectable cancers with a multi-analyte blood test
    Cohen, Joshua D.
    Li, Lu
    Wang, Yuxuan
    Thoburn, Christopher
    Afsari, Bahman
    Danilova, Ludmila
    Douville, Christopher
    Javed, Ammar A.
    Wong, Fay
    Mattox, Austin
    Hruban, Ralph. H.
    Wolfgang, Christopher L.
    Goggins, Michael G.
    Dal Molin, Marco
    Wang, Tian-Li
    Roden, Richard
    Klein, Alison P.
    Ptak, Janine
    Dobbyn, Lisa
    Schaefer, Joy
    Silliman, Natalie
    Popoli, Maria
    Vogelstein, Joshua T.
    Browne, James D.
    Schoen, Robert E.
    Brand, Randall E.
    Tie, Jeanne
    Gibbs, Peter
    Wong, Hui-Li
    Mansfield, Aaron S.
    Jen, Jin
    Hanash, Samir M.
    Falconi, Massimo
    Allen, Peter J.
    Zhou, Shibin
    Bettegowda, Chetan
    Diaz, Luis A., Jr.
    Tomasetti, Cristian
    Kinzler, Kenneth W.
    Vogelstein, Bert
    Lennon, Anne Marie
    Papadopoulos, Nickolas
    [J]. SCIENCE, 2018, 359 (6378) : 926 - +
  • [5] Liquid Biopsies: Genotyping Circulating Tumor DNA
    Diaz, Luis A.
    Bardelli, Alberto
    [J]. JOURNAL OF CLINICAL ONCOLOGY, 2014, 32 (06) : 579 - +
  • [6] Identification of Double-stranded Genomic DNA Spanning All Chromosomes with Mutated KRAS and p53 DNA in the Serum Exosomes of Patients with Pancreatic Cancer
    Kahlert, Christoph
    Melo, Sonia A.
    Protopopov, Alexei
    Tang, Jiabin
    Seth, Sahil
    Koch, Moritz
    Zhang, Jianhua
    Weitz, Juergen
    Chin, Lynda
    Futreal, Andrew
    Kalluri, Raghu
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (07) : 3869 - 3875
  • [7] Exosomes in tumor microenvironment influence cancer progression and metastasis
    Kahlert, Christoph
    Kalluri, Raghu
    [J]. JOURNAL OF MOLECULAR MEDICINE-JMM, 2013, 91 (04): : 431 - 437
  • [8] Discovery of Double-Stranded Genomic DNA in Circulating Exosomes
    Kalluri, Raghu
    LeBleu, Valerie S.
    [J]. TARGETING CANCER, VOL 81, 2016, 2016, 81 : 275 - 280
  • [9] The biology and function of exosomes in cancer
    Kalluri, Raghu
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2016, 126 (04) : 1208 - 1215
  • [10] Extracellular vesicles or free circulating DNA: where to search for BRAF and cKIT mutations?
    Klump, Jennifer
    Phillipp, Ulrike
    Follo, Marie
    Eremin, Anna
    Lehmann, Hannes
    Nestel, Sigrun
    von Bubnoff, Nikolas
    Nazarenko, Irina
    [J]. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2018, 14 (03) : 875 - 882
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