Preparation and Electrochemical Behavior of L-Glutamate Electrochemical Biosensor

被引：255
作者：
Deng, Yan [1 ,2 ]
Wang, Wei [1 ]
Zhang, Liming [1 ,2 ]
Lu, Zhuoxuan [1 ,2 ]
Li, Song [1 ]
Xu, Lijian [1 ]
机构：
[1] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China
[2] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
来源：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2013年 / 9卷 / 02期
关键词：
L-Glutamate; Biosensor; Electrochemical; Nano-Porous Pseudo Carbon Paste Electrode; CARBON NANOTUBES; MAGNETIC NANOPARTICLES; MASS-SPECTROMETRY; ACID; OXIDASE; SYSTEM; CANCER; ZNO;
D O I：
10.1166/jbn.2013.1487
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano-PPCPE exhibits a high selectivity and sensitivity in comparison with the modified CPE. The L-glutamate biosensor showed a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M.
引用
收藏
页码：318 / 321
页数：4
相关论文
共 50 条
[21]   Amperometric L-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase [J].
Liang, Bo ;
Zhang, Shu ;
Lang, Qiaolin ;
Song, Jianxia ;
Han, Lihui ;
Liu, Aihua .
ANALYTICA CHIMICA ACTA, 2015, 884 :83-89
[22]   Highly sensitive glutamate biosensor based on platinum nanoparticles decorated MXene-Ti3C2Tx for L-glutamate determination in foodstuffs [J].
Liu, Jingsi ;
Fan, Yuxia ;
Chen, Gaole ;
Liu, Yuan .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2021, 148
[23]   Characterization of a planar L-glutamate amperometric biosensor immobilized with a photo-crosslinkable polymer membrane [J].
Chang, Ku-Shang ;
Chang, Chen-Kai ;
Chou, Su-Fen ;
Han, Hsei-Chen ;
Chen, Chien-Yuan .
SENSORS AND ACTUATORS B-CHEMICAL, 2007, 122 (01) :195-203
[24]   Development and characterization of choline and L-glutamate biosensor integrated on silicon microprobes for in-vivo monitoring [J].
Frey, Olivier ;
van der Wal, Peter ;
de Rooij, Nico ;
Koudelka-Hep, Milena .
2007 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-16, 2007, :6040-6043
[25]   Evaluation of a thermophile enzyme for a carbon paste amperometric biosensor: L-glutamate dehydrogenase [J].
Jeffries, C ;
Pasco, N ;
Baronian, K ;
Gorton, L .
BIOSENSORS & BIOELECTRONICS, 1997, 12 (03) :225-232
[26]   Rapid and simple preparation of a reagentless glucose electrochemical biosensor [J].
Zheng, Dan ;
Vashist, Sandeep Kumar ;
Al-Rubeaan, Khalid ;
Luong, John H. T. ;
Sheu, Fwu-Shan .
ANALYST, 2012, 137 (16) :3800-3805
[27]   Gravure Printed Electrochemical Biosensor [J].
Reddy, A. S. G. ;
Narakathu, B. B. ;
Atashbar, M. Z. ;
Rebros, M. ;
Rebrosova, E. ;
Joyce, M. K. .
EUROSENSORS XXV, 2011, 25
[28]   Optical biosensor test strip for the screening and direct determination of L-glutamate in food samples [J].
Muslim, Noor Zuhartini Md ;
Ahmad, Musa ;
Heng, Lee Yook ;
Saad, Bahruddin .
SENSORS AND ACTUATORS B-CHEMICAL, 2012, 161 (01) :493-497
[29]   Enzyme-based choline and L-glutamate biosensor electrodes on silicon microprobe arrays [J].
Frey, O. ;
Holtzman, T. ;
McNamara, R. M. ;
Theobald, D. E. H. ;
van der Wal, P. D. ;
de Rooij, N. F. ;
Dalley, J. W. ;
Koudelka-Hep, M. .
BIOSENSORS & BIOELECTRONICS, 2010, 26 (02) :477-484
[30]   Construction of minitype glutamate sensor for in vivo monitoring of l-glutamate in plant [J].
Lin, Ye ;
Yang, Lingfen ;
Ma, Ying ;
Ye, Jianshan .
MICROCHEMICAL JOURNAL, 2023, 188
12345