Rapid Separation of Human Serum Proteins by Microchip Electrophoresis

This study focused on the separation of human serum proteins by microchip electrophoresis. It was illustrated the sample injection time and applied voltages significantly affected the final detection and quantitative analysis on a cross microchip. The developed background electrolyte (BGE) was consisted of 100 mmol/L H3BO3, 50 mmol/L NaCl, 5% dextran (pH 8.3 adjusted by NaOH). Five protein fractions, alpha 1-, alpha 2-, beta- gamma-globulins and albumin in human serum, could be separated, moreover, their effective mobilities were estimated in the range of 5.15 similar to 47.2 X 10(-5) cm(2)V(-1)s(-1) in this buffer system. The injection and separation were finished within 2 min in the chip, which was faster than traditional capillary zone electrophoresis.