Crossovers trigger a remodeling of meiotic chromosome axis composition that is linked to two-step loss of sister chromatid cohesion

被引:119
作者
Martinez-Perez, Enrique [1 ]
Schvarzstein, Mara [2 ]
Barroso, Consuelo [1 ]
Lightfoot, James [1 ]
Dernburg, Abby F. [3 ,4 ]
Villeneuve, Anne M. [2 ]
机构
[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA
[3] EO Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA
[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
基金
英国生物技术与生命科学研究理事会;
关键词
Meiosis; chromosome axes; crossover; sister chromatid cohesion; chromosome remodeling; crossover interference;
D O I
10.1101/gad.1694108
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Segregation of homologous chromosomes during meiosis depends on linkages ( chiasmata) created by crossovers and on selective release of a subset of sister chromatid cohesion at anaphase I. During Caenorhabditis elegans meiosis, each chromosome pair forms a single crossover, and the position of this event determines which chromosomal regions will undergo cohesion release at anaphase I. Here we provide insight into the basis of this coupling by uncovering a large-scale regional change in chromosome axis composition that is triggered by crossovers. We show that axial element components HTP-1 and HTP-2 are removed during late pachytene, in a crossover-dependent manner, from the regions that will later be targeted for anaphase I cohesion release. We demonstrate correspondence in position and number between chiasmata and HTP-1/2-depleted regions and provide evidence that HTP-1/2 depletion boundaries mark crossover sites. In htp-1 mutants, diakinesis bivalents lack normal asymmetrical features, and sister chromatid cohesion is prematurely lost during the meiotic divisions. We conclude that HTP-1 is central to the mechanism linking crossovers with late-prophase bivalent differentiation and defines the domains where cohesion will be protected until meiosis II. Further, we discuss parallels between the pattern of HTP-1/2 removal in response to crossovers and the phenomenon of crossover interference.
引用
收藏
页码:2886 / 2901
页数:16
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