The Effect of Guanidine Hydrochloride on Phase Diagram of PEG-Phosphate Aqueous Two-Phase System

被引:0
|
作者
Rahimpour, Farshad [1 ]
Pirdashti, Mohsen [1 ]
机构
[1] Razi Univ, Dept Chem Engn, Biotechnol Res Lab, Fac Engn, Kermanshah 6714967346, Iran
关键词
Aqueous two-phase system; guanidine hydrochloride; partition coefficient; phase diagram;
D O I
暂无
中图分类号
TP39 [计算机的应用];
学科分类号
081203 ; 0835 ;
摘要
This report focus on phase behavior of polyethylene glycol (PEG)4000/ phosphate/ guanidine hydrochloride/ water system at different guanidine hydrochloride concentrations and pH. The binodal of the systems was displaced toward higher concentrations of the components with increasing guanidine hydrochloride concentrations. The partition coefficient of guanidine hydrochloride was near unity and increased with decreasing pH and increasing PEG/salt (%w/w) ratio.
引用
收藏
页码:124 / 128
页数:5
相关论文
共 50 条
  • [1] Hydrodynamics of PEG-phosphate aqueous two-phase systems in a J-type multilayer countercurrent chromatograph
    Al-Marzouqi, I
    Levy, MS
    Lye, GJ
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2005, 28 (09) : 1311 - 1332
  • [2] Phase Diagram of Aqueous Two-Phase System (ATPS) Composed of Polyethylene Glycol (PEG) and Gelatin
    Ma, Chao
    Chen, Xiao Dong
    Kong, Yinqiu
    Che, Liming
    ADVANCES IN CHEMISTRY RESEARCH II, PTS 1-3, 2012, 554-556 : 286 - +
  • [3] Scale-up of recombinant cutinase recovery by whole broth extraction with PEG-phosphate aqueous two-phase
    Costa, MJL
    Cunha, MT
    Cabral, JMS
    Aires-Barros, MR
    BIOSEPARATION, 2000, 9 (04) : 231 - 238
  • [4] Purification of α-toxin from Clostridium perfringens type A in PEG-phosphate aqueous two-phase systems:: a factorial study
    Cavalcanti, Maria T. H.
    Porto, Tatiana S.
    Neto, Benicio B.
    Lima-Filho, Jose L.
    Porto, Ana L. F.
    Pessoa, Adalberto, Jr.
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2008, 83 (02) : 158 - 162
  • [5] Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems
    Bandmann, N
    Collet, E
    Leijen, J
    Uhlén, M
    Veide, A
    Nygren, PÅ
    JOURNAL OF BIOTECHNOLOGY, 2000, 79 (02) : 161 - 172
  • [6] Extraction of fibrinolytic proteases from Streptomyces sp DPUA1576 using PEG-phosphate aqueous two-phase systems
    de Medeiros e Silva, Germana Michelle
    Viana Marques, Daniela de Araujo
    Porto, Tatiana Souza
    Lima Filho, Jose Luiz
    Couto Teixeira, Jose Antonio
    Pessoa-Junior, Adalberto
    Figueiredo Porto, Ana Lucia
    FLUID PHASE EQUILIBRIA, 2013, 339 : 52 - 57
  • [7] Effects of polymer pH and molar mass on the PEG/phosphate aqueous two-phase system
    Padilha, Giovana da Silva
    Ferreira, Juliana Ferrari
    Alegre, Ranulfo Monte
    Tambourgi, Elias Basile
    ACTA SCIENTIARUM-TECHNOLOGY, 2011, 33 (01) : 1 - 4
  • [8] Revisiting the driving force inducing phase separation in PEG-phosphate aqueous biphasic systems
    Bonnassieux, Sophie
    Pandya, Raj
    Skiba, Dhyllan Adan
    Degoulange, Damien
    Petit, Dorothee
    Seem, Peter
    Cowburn, Russel P.
    Gallant, Betar M.
    Grimaud, Alexis
    FARADAY DISCUSSIONS, 2024, 253 (00) : 181 - 192
  • [9] Measurement and correlation of partition coefficients of urokinase in aqueous two-phase peg potassium phosphate system
    Zhu, ZQ
    Li, MA
    Mei, LH
    Han, ZX
    CHINESE JOURNAL OF CHEMICAL ENGINEERING, 1996, 4 (01) : 19 - 27
  • [10] Response surface methodology for the evaluation of guanidine hydrochloride partitioning in polymer-salt aqueous two-phase system
    Mohsen Pirdashti
    Kamyar Movagharnejad
    Abbas Ali Rostami
    Behnia Shahrokhi
    Korean Journal of Chemical Engineering, 2017, 34 : 2033 - 2042