The Effect of Guanidine Hydrochloride on Phase Diagram of PEG-Phosphate Aqueous Two-Phase System

被引：0

作者：

Rahimpour, Farshad ^{[1
]}

Pirdashti, Mohsen ^{[1
]}

机构：

[1] Razi Univ, Dept Chem Engn, Biotechnol Res Lab, Fac Engn, Kermanshah 6714967346, Iran

来源：

PROCEEDINGS OF WORLD ACADEMY OF SCIENCE, ENGINEERING AND TECHNOLOGY, VOL 23 | 2007年 / 23卷

关键词：

Aqueous two-phase system; guanidine hydrochloride; partition coefficient; phase diagram;

D O I：

暂无

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

This report focus on phase behavior of polyethylene glycol (PEG)4000/ phosphate/ guanidine hydrochloride/ water system at different guanidine hydrochloride concentrations and pH. The binodal of the systems was displaced toward higher concentrations of the components with increasing guanidine hydrochloride concentrations. The partition coefficient of guanidine hydrochloride was near unity and increased with decreasing pH and increasing PEG/salt (%w/w) ratio.

引用

页码：124 / 128

页数：5

共 15 条

[1] ALBARTSSON PA, 1986, PARTITION CELL PARTI
[2] Effect of polyethylene glycol on the liquid-liquid phase transition in aqueous protein solutions
Annunziata, O
Asherie, N
Lomakin, A
Pande, J
Ogun, O
Benedek, GB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (22) : 14165 - 14170
[3] CARBAMYLATION OF GLOBIN IN ELECTROPHORESIS AND CHROMATOGRAPHY IN PRESENCE OF UREA
CEJKA, J
VODRAZKA, Z
SALAK, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 154 (03) : 589 - +
[4] Protein refolding for industrial processes
Clark, ED
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (02) : 202 - 207
[5] Estape D, 1996, BIOTECHNOL TECH, V10, P481
[6] PROTEIN REFOLDING USING AQUEOUS 2-PHASE SYSTEMS
FORCINITI, D
[J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 668 (01) : 95 - 100
[7] CYANATE FORMATION IN SOLUTIONS OF UREA .1. CALCULATION OF CYANATE CONCENTRATIONS AT DIFFERENT TEMPERATURE AND PH
HAGEL, P
GERDING, JJT
FIEGGEN, W
BLOEMENDAL, H
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 243 (03) : 366 - +
[8] The new experimental data and a new thermodynamic model based on group contribution for correlation liquid-liquid equilibria in aqueous two-phase systems of PEG and (K2HPO4 or Na2SO4)
Haghtalab, A
Mokhtarani, B
[J]. FLUID PHASE EQUILIBRIA, 2004, 215 (02) : 151 - 161
[9] LARGE-SCALE, IN-SITU ISOLATION OF PERIPLASMIC IGF-I FROM ESCHERICHIA-COLI
HART, RA
LESTER, PM
REIFSNYDER, DH
OGEZ, JR
BUILDER, SE
[J]. BIO-TECHNOLOGY, 1994, 12 (11): : 1113 - 1117
[10] Optimizing refolding and recovery of active recombinant Bacillus halodurans xylanase in polymer-salt aqueous two-phase system using surface response analysis
Rahimpour, Farshad
Mamo, Gashaw
Feyzi, Farzaneh
Maghsoudi, Saeid
Hatti-Kaul, Rajni
[J]. JOURNAL OF CHROMATOGRAPHY A, 2007, 1141 (01) : 32 - 40

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