Vertical distribution of bacterioplankton in Lake Averno in relation to water chemistry

被引:12
作者
Paganin, Patrizia [1 ]
Chiarini, Luigi [1 ]
Bevivino, Annamaria [1 ]
Dalmastri, Claudia [1 ]
Farcomeni, Alessio [2 ]
Izzo, Giulio [3 ]
Signorini, Antonella [3 ]
Varrone, Cristiano [3 ]
Tabacchioni, Silvia [1 ]
机构
[1] ENEA CR Casaccia, Tech Unit Sustainable Dev & Innovat Agroind Syst, I-00123 Rome, Italy
[2] Univ Roma La Sapienza, Stat Sect, Dept Publ Hlth & Infect Dis, Rome, Italy
[3] ENEA CR Casaccia, Tech Unit Renewable Energy Resources, I-00123 Rome, Italy
关键词
Bacteria; Archaea; denaturing gradient gel electrophoresis; nonlinear multidimensional scaling; lake water column; environmental parameters; 16S RIBOSOMAL-RNA; GRADIENT GEL-ELECTROPHORESIS; BACTERIAL COMMUNITY COMPOSITION; MARINE BENTHIC ARCHAEA; MICROBIAL COMMUNITIES; MEROMICTIC LAKE; PHYLOGENETIC DIVERSITY; MONO LAKE; ASSEMBLAGES; HETEROGENEITY;
D O I
10.1111/1574-6941.12048
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The effects of environmental factors on bacterioplankton distribution along the water column of Lake Averno (Naples, Italy) have been investigated by means of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments, and multivariate analysis applied to molecular data and physico-chemical parameters. Bacterial richness, estimated from DGGE profiles, remains constant throughout the water column, whereas archaeal richness increases with depth. Moreover, archaeal richness was significantly correlated to most of the measured abiotic variables, whereas bacterial richness did not. Analysis of sequences from DGGE bands revealed that the dominant bacterial populations belong to Alphaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Bacteroidetes and Firmicutes, whereas sequences of the archaeal DGGE bands are affiliated to Methanomicrobiales and Methanosarcinales orders. Moreover, bacterial sequences affiliated to Proteobacteria and Bacteroidetes do not group closely to typical freshwater lineages/clades/tribes within these phyla. Bacterioplankton distribution along the water column was apparently correlated with the vertical gradient of physico-chemical parameters as a statistically significant relationship between most of them with grouping of specific taxonomic units was observed.
引用
收藏
页码:176 / 188
页数:13
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