The microstructure of collagen type I gel cross-linked with gold nanoparticles

被引：42

作者：

Schuetz, Thomas ^{[1
]}

Richmond, Nathan ^{[1
]}

Harmon, Marianne E. ^{[2
]}

Schuetz, Joseph ^{[2
]}

Castaneda, Luciano ^{[1
]}

Slowinska, Katarzyna ^{[1
]}

机构：

[1] Calif State Univ Long Beach, Dept Chem & Biochem, Long Beach, CA 90840 USA

[2] Aerosp Corp, Micro Nano Technol Dept, El Segundo, CA 90245 USA

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2013年 / 101卷

基金：

美国国家卫生研究院;

关键词：

Collagen gel; Au nanoparticles; Multivalent cross-linkers; Microstructure; TIME-OF-FLIGHT; MECHANICAL-PROPERTIES; CHONDROITIN SULFATE; LINKING; DELIVERY; SCAFFOLD; MATRICES; SPONGES; TISSUES; SYSTEMS;

D O I：

10.1016/j.colsurfb.2012.06.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Scanning electron microscopy, transmission electron microscopy, rheometry, and electrochemistry were used to provide insight into the microstructure of collagen type I gel (1%, w/v) modified with the tiopronin-protected (N-(2-mercaptopropionyl)glycine) gold nanoparticles (TPAu), a multivalent crosslinker. The cross-linking reaction, performed via EDC (1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide) coupling, results in compliant, mechanically stable and continuous gels. The gels contain unusual interconnected collagen-TPAu particles. Electrochemical measurements of 4-hydroxy-(2,2,6,6-tetramethylpiperidine-1-oxyl) (4HT) diffusion within the gel reveal that the gel hindrance is nearly independent of the TPAu concentration. The properties of the collagen-TPAu gel make it suitable for potential biomedical applications, such as delivery of small molecule drugs. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：118 / 125

页数：8

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