Glioblastomas (GBMs) are the most common and lethal primary tumors of the central nervous system with high level of recurrence despite aggressive therapy. Tumor-associated proteins/peptides may appear in the plasma of these patients as a result of disruption of the blood-brain barrier in them, raising the scope for development of plasma-based tests for diagnosis and monitoring the disease. With this objective, we analyzed the levels of proteins present in the plasma from GBM patients using an iTRAQ based LC-MS/MS approach. Analysis with pooled plasma specimens from the patient and healthy control samples revealed high confidence identification of 296 proteins, of which 61 exhibited a fold-change >= 1.5 in the patient group. Forty-eight of them contained signal sequence. A majority have been reported in the differentially expressed transcript or protein profile of GBM tissues; 6 have been previously studied as plasma biomarkers for GBM and 16 for other types of cancers. Altered levels of three representative proteins-ferritin light chain (FTL), S100A9, and carnosinase 1 (CNDP1)-were verified by ELISA in a test set of ten individual plasma specimens. FTL is an inflammation marker also implicated in cancer, S100A9 is an important member of the Ca2+ signaling cascade reported to be altered in GBM tissue, and CNDP1 has been reported for its role in the regulation of the levels of carnosine, implicated as a potential drug for GBM. These and other proteins in the dataset may form useful starting points for further clinical investigations for the development of plasma-based biomarker panels for GBM.
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
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Univ Michigan, Ctr Computat Med & Bioinformat, Ann Arbor, MI 48109 USAInst Syst Biol, Seattle, WA 98109 USA
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Univ So Calif, Dept Med, Los Angeles, CA USAInst Syst Biol, Seattle, WA 98109 USA
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Univ So Calif, Dept Med, Los Angeles, CA USAInst Syst Biol, Seattle, WA 98109 USA
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ETH Swiss Fed Inst Technol, Dept Biol, Inst Mol Syst Biol, Zurich, SwitzerlandInst Syst Biol, Seattle, WA 98109 USA
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ETH Swiss Fed Inst Technol, Dept Biol, Inst Mol Syst Biol, Zurich, SwitzerlandInst Syst Biol, Seattle, WA 98109 USA
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Swedish Med Ctr, Swedish Neurosci Inst, Seattle, WA USA
Zhejiang Univ, Syst Biol Div, Zhejiang California Int Nanosyst Inst ZCNI, Hangzhou 310003, Zhejiang, Peoples R ChinaInst Syst Biol, Seattle, WA 98109 USA
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Johns Hopkins Univ, Dept Pathol, Baltimore, MD USAInst Syst Biol, Seattle, WA 98109 USA
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Aebersold, Ruedi
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ETH Swiss Fed Inst Technol, Dept Biol, Inst Mol Syst Biol, Zurich, SwitzerlandInst Syst Biol, Seattle, WA 98109 USA
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
Farrah, Terry
Deutsch, Eric W.
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
Deutsch, Eric W.
Omenn, Gilbert S.
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Inst Syst Biol, Seattle, WA 98109 USA
Univ Michigan, Ctr Computat Med & Bioinformat, Ann Arbor, MI 48109 USAInst Syst Biol, Seattle, WA 98109 USA
Omenn, Gilbert S.
Campbell, David S.
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
Campbell, David S.
Sun, Zhi
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
Sun, Zhi
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
Bletz, Julie A.
Mallick, Parag
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Univ So Calif, Dept Med, Los Angeles, CA USAInst Syst Biol, Seattle, WA 98109 USA
Mallick, Parag
Katz, Jonathan E.
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Univ So Calif, Dept Med, Los Angeles, CA USAInst Syst Biol, Seattle, WA 98109 USA
Katz, Jonathan E.
Malmstroem, Johan
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ETH Swiss Fed Inst Technol, Dept Biol, Inst Mol Syst Biol, Zurich, SwitzerlandInst Syst Biol, Seattle, WA 98109 USA
Malmstroem, Johan
Ossola, Reto
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ETH Swiss Fed Inst Technol, Dept Biol, Inst Mol Syst Biol, Zurich, SwitzerlandInst Syst Biol, Seattle, WA 98109 USA
Ossola, Reto
Watts, Julian D.
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
Watts, Julian D.
Lin, Biaoyang
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Swedish Med Ctr, Swedish Neurosci Inst, Seattle, WA USA
Zhejiang Univ, Syst Biol Div, Zhejiang California Int Nanosyst Inst ZCNI, Hangzhou 310003, Zhejiang, Peoples R ChinaInst Syst Biol, Seattle, WA 98109 USA
Lin, Biaoyang
Zhang, Hui
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Johns Hopkins Univ, Dept Pathol, Baltimore, MD USAInst Syst Biol, Seattle, WA 98109 USA
Zhang, Hui
Moritz, Robert L.
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Inst Syst Biol, Seattle, WA 98109 USAInst Syst Biol, Seattle, WA 98109 USA
Moritz, Robert L.
Aebersold, Ruedi
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ETH Swiss Fed Inst Technol, Dept Biol, Inst Mol Syst Biol, Zurich, SwitzerlandInst Syst Biol, Seattle, WA 98109 USA