Gene expression analysis in human monocytes, monocyte-derived dendritic cells, and α-galactosylceramide-pulsed monocyte-derived dendritic cells

被引：8
作者：
Lapteva, N
Nieda, M
Ando, Y
Nicol, A
Ide, K
Yamaura, A
Hatta-Ohashi, Y
Egawa, K
Juji, T
Tokunaga, K
机构：
[1] MEDINET Co Ltd, Lab Mol Immunol, Setagaya Ku, Tokyo 1580095, Japan
[2] Univ Tokyo, Grad Sch Med, Dept Human Genet, Tokyo, Japan
[3] Russian Acad Sci, Inst Cytol & Genet, Lab Genome Struct, Novosibirsk 630090, Russia
[4] Japanese Red Cross Cent Blood Ctr, Res Sect 1, Tokyo, Japan
[5] Queensland Inst Med Res, Brisbane, Qld 4006, Australia
[6] Univ Queensland, Dept Med, Brisbane, Qld 4000, Australia
[7] Olympus Opt Co Ltd, Tokyo, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 289卷 / 02期
关键词：
alpha-galactosylceramide; dendritic cells; microarray; RNase A; collapsin response mediator protein;
D O I：
10.1006/bbrc.2001.6003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In vitro proliferation and functional activation of V alpha 24NKT cells following stimulation with alpha -galactosylceramide (alpha -GalCer)-pulsed dendritic cells (DCs) have been observed. Because little is known about the molecular events on DCs following interaction with alpha -GalCer, we performed gene expression profiling of 2400 genes in monocytes and monocyte-derived immature DCs pulsed with alpha -GalCer (alpha -GalCer-imDCs). Overall, the expression levels of 48 genes were up-regulated and 28 were down-regulated in alpha -GalCer-imDCs. Semiquantitative RT-PCR analysis on monocytes, imDCs, alpha -GalCer-imDCs, and mature DCs confirmed the up- and down-regulation of the mRNA expression levels of 28 selected genes. Notably, we identified the specific up-regulation of mRNA expression levels of ribonuclease A and collapsin response mediator protein upon the stimulation of imDC with alpha -GalCer, suggesting a novel immunomodulating effect of alpha -GalCer on imDCs. In this study, we used imDCs prepared by culturing of monocytes with GM-CSF and IL-4 for 5 days and mDCs prepared by further culturing of imDCs with TNF alpha for two extra days. (C) 2001 Elsevier Science.
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页码：531 / 538
页数：8
相关论文
共 27 条
[1]  
Austyn J M, 1992, Semin Immunol, V4, P227
[2]   Immunobiology of dendritic cells [J].
Banchereau, J ;
Briere, F ;
Caux, C ;
Davoust, J ;
Lebecque, S ;
Liu, YT ;
Pulendran, B ;
Palucka, K .
ANNUAL REVIEW OF IMMUNOLOGY, 2000, 18 :767-+
[3]  
Barzilai A, 2000, ADV RES NEURODEGENER, V8, P59
[4]   The ribonuclease A superfamily: general discussion [J].
Beintema, JJ ;
Kleineidam, RG .
CELLULAR AND MOLECULAR LIFE SCIENCES, 1998, 54 (08) :825-832
[5]  
Bubeník J, 2001, INT J ONCOL, V18, P475
[6]   GM-CSF AND TNF-ALPHA COOPERATE IN THE GENERATION OF DENDRITIC LANGERHANS CELLS [J].
CAUX, C ;
DEZUTTERDAMBUYANT, C ;
SCHMITT, D ;
BANCHEREAU, J .
NATURE, 1992, 360 (6401) :258-261
[7]   Peptide-pulsed dendritic cells induce antigen-specific, CTL-mediated protective tumor immunity [J].
Celluzzi, CM ;
Mayordomo, JI ;
Storkus, WJ ;
Lotze, MT ;
Falo, LD .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (01) :283-287
[8]   Maturation of human monocyte-derived dendritic cells studied by microarray hybridization [J].
Dietz, AB ;
Bulur, PA ;
Knutson, GJ ;
Matasic, R ;
Vuk-Pavlovic, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (03) :731-738
[9]   VACCINATION WITH CYTOTOXIC T-LYMPHOCYTE EPITOPE-CONTAINING PEPTIDE PROTECTS AGAINST A TUMOR-INDUCED BY HUMAN PAPILLOMAVIRUS TYPE-16-TRANSFORMED CELLS [J].
FELTKAMP, MCW ;
SMITS, HL ;
VIERBOOM, MPM ;
MINNAAR, RP ;
DEJONGH, BM ;
DRIJFHOUT, JW ;
TERSCHEGGET, J ;
MELIEF, CJM ;
KAST, WM .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (09) :2242-2249
[10]   Identification of genes specifically expressed in human activated and mature dendritic cells through serial analysis of gene expression [J].
Hashimoto, S ;
Suzuki, T ;
Nagai, S ;
Yamashita, T ;
Toyoda, N ;
Matsushima, K .
BLOOD, 2000, 96 (06) :2206-2214
123