A comparison of cytogenetic studies and flow cytometry in breast carcinomas

被引:0
作者
Steinarsdottir, M
Jonasson, JG
Petursdottir, I
Sigurdsson, H
Ogmundsdottir, HM
机构
[1] UNIV ICELAND,DEPT PATHOL,REYKJAVIK,ICELAND
[2] ICLANDIC CANC SOC,MOL & CELL BIOL RES LAB,REYKJAVIK,ICELAND
[3] UNIV HOSP ICELAND,DEPT ONCOL,REYKJAVIK,ICELAND
来源
CYTOMETRY | 1997年 / 28卷 / 04期
关键词
cytogenetics; flow cytometry; breast carcinomas;
D O I
10.1002/(SICI)1097-0320(19970801)28:4<323::AID-CYTO8>3.0.CO;2-C
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we compared genetic instability in 70 breast carcinomas analyzed by two different methods, cytogenetics and flow cytometry. This comparison showed that each method has its strengths and weaknesses. Flow cytometry detected aneuploidy in 60% of cases, but missed most of the cytogenetically near-diploid clones and clones with simple chromosomal changes. Cytogenetics revealed chromosomal abnormalities in 50% of the samples. Simple chromosomal changes and multiploidy were readily detected by this method, but some of the clones with a high DNA index: by flow cytometry were missed. The two methods gave corresponding results in the majority of cases (54%). In 17 cases, both methods detected matching abnormal clones (r = 0.93) but the DNA index was higher than predicted by the chromosome numbers. Most of the discrepancies might be explained by tumor heterogeneity and insufficient numbers of cells available for cytogenetic analyses. In seven cases, single-cell abnormalities were found that corresponded to a pow cytometry peak. Multiclonality was present in 25% of aneuploid tumors. No association was found between metaphases in cytogenetic preparations and increased S-phase fraction of the tumors, but aneuploid tumors had a significantly higher proliferation rate. Pooling data from both methods demonstrated that the majority of our samples were aneuploid (74%). (C) 1997 Wiley-Liss, Inc.
引用
收藏
页码:323 / 328
页数:6
相关论文
共 32 条
  • [1] BONSING BA, 1993, CANCER, V71, P382, DOI 10.1002/1097-0142(19930115)71:2<382::AID-CNCR2820710219>3.0.CO
  • [2] 2-9
  • [3] Metaphase cytogenetics and DNA flow cytometry with analysis of S-phase fraction in prostate cancer: Influence on prognosis
    Bratt, O
    Anderson, H
    BakJensen, E
    Baldetorp, B
    Lundgren, R
    [J]. UROLOGY, 1996, 47 (02) : 218 - 224
  • [4] SOMATIC GENETIC CHANGES IN HUMAN BREAST-CANCER
    DEVILEE, P
    CORNELISSE, CJ
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 1994, 1198 (2-3): : 113 - 130
  • [5] DRESSLER LG, 1993, CANCER, V72, P2033, DOI 10.1002/1097-0142(19930915)72:6<2033::AID-CNCR2820720640>3.0.CO
  • [6] 2-J
  • [7] CHARACTERIZATION OF CHROMOSOMAL-ANOMALIES IN HUMAN BREAST-CANCER - A COMPARISON OF 30 PARADIPLOID CASES WITH FEW CHROMOSOME CHANGES
    DUTRILLAUX, B
    GERBAULTSEUREAU, M
    ZAFRANI, B
    [J]. CANCER GENETICS AND CYTOGENETICS, 1990, 49 (02) : 203 - 217
  • [8] BREAST-CANCER GENETIC EVOLUTION .1. DATA FROM CYTOGENETICS AND DNA CONTENT
    DUTRILLAUX, B
    GERBAULTSEUREAU, M
    REMVIKOS, Y
    ZAFRANI, B
    PRIEUR, M
    [J]. BREAST CANCER RESEARCH AND TREATMENT, 1991, 19 (03) : 245 - 255
  • [9] METHOD FOR ANALYSIS OF CELLULAR DNA CONTENT OF PARAFFIN-EMBEDDED PATHOLOGICAL MATERIAL USING FLOW-CYTOMETRY
    HEDLEY, DW
    FRIEDLANDER, ML
    TAYLOR, IW
    RUGG, CA
    MUSGROVE, EA
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1983, 31 (11) : 1333 - 1335
  • [10] JONASSON JG, 1994, VIRCHOWS ARCH, V425, P349