Analytical Performance Evaluation of New DESI Enhancements for Targeted Drug Quantification in Tissue Sections

被引：8

作者：

Fresnais, Margaux ^{[1
]}

Liang, Siwen ^{[1
]}

Breitkopf, Marius ^{[1
]}

Lindner, Joshua Raoul ^{[1
]}

Claude, Emmanuelle ^{[2
]}

Pringle, Steven ^{[2
]}

Levkin, Pavel A. ^{[3
,4
]}

Demir, Konstantin ^{[3
]}

Benzel, Julia ^{[5
,6
]}

Sundheimer, Julia ^{[5
,6
]}

Statz, Britta ^{[5
,6
]}

Pajtler, Kristian W. ^{[5
,6
,7
]}

Pfister, Stefan M. ^{[5
,6
,7
]}

Haefeli, Walter E. ^{[1
]}

Burhenne, Juergen ^{[1
]}

Longuespee, Remi ^{[1
]}

机构：

[1] Heidelberg Univ Hosp, Dept Clin Pharmacol & Pharmacoepidemiol, Neuenheimer Feld 410, D-69120 Heidelberg, Germany

[2] Waters, Stamford Ave,Altrincham Rd, Wilmslow SK9 4AX, Cheshire, England

[3] Aquarray, Hermann von Helmholtz Pl 6, D-76344 Eggenstein Leopoldshafen, Germany

[4] Karlsruhe Inst Technol KIT, Inst Biol & Chem Syst Funct Mol Syst IBCS FMS, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

[5] Hopp Childrens Canc Ctr Heidelberg KiTZ, Neuenheimer Feld 430, D-69120 Heidelberg, Germany

[6] German Canc Res Ctr, German Canc Consortium DKTK, Div Pediat Neurooncol, Neuenheimer Feld 280, D-69120 Heidelberg, Germany

[7] Heidelberg Univ Hosp, Dept Pediat Hematol Oncol & Immunol, D-69120 Heidelberg, Germany

来源：

PHARMACEUTICALS | 2022年 / 15卷 / 06期

关键词：

desorption electrospray ionization; mass spectrometry; drug; quantification; profiling; IMAGING MASS-SPECTROMETRY; TIME-OF-FLIGHT; PLASMA; METABOLITES; OCTREOTIDE; LIVER;

D O I：

10.3390/ph15060694

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Desorption/ionization (DI)-mass spectrometric (MS) methods offer considerable advantages of rapidity and low-sample input for the analysis of solid biological matrices such as tissue sections. The concept of desorption electrospray ionization (DESI) offers the possibility to ionize compounds from solid surfaces at atmospheric pressure, without the addition of organic compounds to initiate desorption. However, severe drawbacks from former DESI hardware stability made the development of assays for drug quantification difficult. In the present study, the potential of new prototype source setups (High Performance DESI Sprayer and Heated Transfer Line) for the development of drug quantification assays in tissue sections was evaluated. It was demonstrated that following dedicated optimization, new DESI XS enhancements present promising options regarding targeted quantitative analyses. As a model compound for these developments, ulixertinib, an inhibitor of extracellular signal-regulated kinase (ERK) 1 and 2 was used.

引用

页数：18

共 52 条

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