Crystal structure of family 4 uracil-DNA glycosylase from Sulfolobus tokodaii and a function of tyrosine 170 in DNA binding

被引：9
|
作者：
Kawai, Akito [1 ]
Higuchi, Shigesada [1 ]
Tsunoda, Masaru [3 ]
Nakamura, Kazuo T. [2 ]
Yamagata, Yuriko [4 ]
Miyamoto, Shuichi [1 ]
机构：
[1] Sojo Univ, Fac Pharmaceut Sci, Nishi Ku, Kumamoto 8600082, Japan
[2] Showa Univ, Sch Pharm, Shinagawa Ku, Tokyo 1428555, Japan
[3] Iwaki Meisei Univ, Fac Pharm, Iwaki, Fukushima 9708551, Japan
[4] Kumamoto Univ, Grad Sch Pharmaceut Sci, Chuo Ku, Kumamoto 8620973, Japan
来源：
FEBS LETTERS | 2015年 / 589卷 / 19期
关键词：
Uracil-DNA glycosylase; Crystal structure; Leucine-intercalation loop; Sulfolobus tokodaii; BASE EXCISION-REPAIR; SPECIFICITY;
D O I：
10.1016/j.febslet.2015.08.019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Uracil-DNA glycosylases (UDGs) excise uracil from DNA by catalyzing the N-glycosidic bond hydrolysis. Here we report the first crystal structures of an archaeal UDG (stoUDG). Compared with other UDGs, stoUDG has a different structure of the leucine-intercalation loop, which is important for DNA binding. The stoUDG-DNA complex model indicated that Leu169, Tyr170, and Asn171 in the loop are involved in DNA intercalation. Mutational analysis showed that Tyr170 is critical for substrate DNA recognition. These results indicate that Tyr170 occupies the intercalation site formed after the structural change of the leucine-intercalation loop required for the catalysis. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2675 / 2682
页数：8
相关论文
共 50 条
  • [31] A structure change-induced fluorescent biosensor for uracil-DNA glycosylase activity detection based on the substrate preference of Lambda exonuclease
    Sun, Jianbo
    Li, Changjiang
    Hu, Yuqiang
    Ding, Yawen
    Wu, Tongbo
    TALANTA, 2022, 243
  • [32] New insights on the role of the γ-herpesvirus uracil-DNA glycosylase leucine loop revealed by the structure of the Epstein-Barr virus enzyme in complex with an inhibitor protein
    Geoui, Thibault
    Buisson, Marlyse
    Tarbouriech, Nicolas
    Burmeister, Wim Pascal
    JOURNAL OF MOLECULAR BIOLOGY, 2007, 366 (01) : 117 - 131
  • [33] Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua)
    Moe, E
    Leiros, I
    Riise, EK
    Olufsen, M
    Lane, O
    Smalås, AO
    Willassen, NP
    JOURNAL OF MOLECULAR BIOLOGY, 2004, 343 (05) : 1221 - 1230
  • [34] Characterization of Heat-labile Uracil-DNA Glycosylase from Oncorhynchus mykiss and its Application for Carry-over Contamination Control in RT-qPCR
    Huang, Qingyuan
    Zhang, Yaqi
    Hu, Wenhao
    Chen, Keqi
    Zhang, Jian
    Luo, Zhidan
    Lu, Chen
    PROTEIN AND PEPTIDE LETTERS, 2024, 31 (03) : 169 - 177
  • [35] CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE URACIL-DNA GLYCOSYLASE DNA-REPAIR ENZYME FROM HERPES-SIMPLEX VIRUS TYPE-1
    SAVVA, R
    PEARL, LH
    JOURNAL OF MOLECULAR BIOLOGY, 1993, 234 (03) : 910 - 912
  • [36] Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway
    Grippon, Seden
    Zhao, Qiyuan
    Robinson, Tom
    Marshall, Jacqueline J. T.
    O'Neill, Rory J.
    Manning, Hugh
    Kennedy, Gordon
    Dunsby, Christopher
    Neil, Mark
    Halford, Stephen E.
    French, Paul M. W.
    Baldwin, Geoff S.
    NUCLEIC ACIDS RESEARCH, 2011, 39 (07) : 2593 - 2603
  • [37] The Role of Active-Site Residues Phe98, His239, and Arg243 in DNA Binding and in the Catalysis of Human Uracil-DNA Glycosylase SMUG1
    Iakovlev, Danila A.
    Alekseeva, Irina V.
    Vorobjev, Yury N.
    Kuznetsov, Nikita A.
    Fedorova, Olga S.
    MOLECULES, 2019, 24 (17):
  • [38] Characterization of heat-labile uracil-DNA glycosylase from Psychrobacter sp HJ147 and its application to the polymerase chain reaction
    Lee, Mi Sun
    Kim, Gun A.
    Seo, Moo Seok
    Lee, Jung-Hyun
    Kwon, Suk-Tae
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2009, 52 : 167 - 175
  • [39] Crystal structure and DNA cleavage mechanism of the restriction DNA glycosylase R.CcoLI from Campylobacter coli
    Miyazono, Ken-ichi
    Wang, Delong
    Ito, Tomoko
    Tanokura, Maseru
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [40] Molecular cloning, sequencing, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346
    Jaeger, S
    Schmuck, R
    Sobek, H
    EXTREMOPHILES, 2000, 4 (02) : 115 - 122
12345