Crystal structure of family 4 uracil-DNA glycosylase from Sulfolobus tokodaii and a function of tyrosine 170 in DNA binding

被引：9

作者：

Kawai, Akito ^{[1
]}

Higuchi, Shigesada ^{[1
]}

Tsunoda, Masaru ^{[3
]}

Nakamura, Kazuo T. ^{[2
]}

Yamagata, Yuriko ^{[4
]}

Miyamoto, Shuichi ^{[1
]}

机构：

[1] Sojo Univ, Fac Pharmaceut Sci, Nishi Ku, Kumamoto 8600082, Japan

[2] Showa Univ, Sch Pharm, Shinagawa Ku, Tokyo 1428555, Japan

[3] Iwaki Meisei Univ, Fac Pharm, Iwaki, Fukushima 9708551, Japan

[4] Kumamoto Univ, Grad Sch Pharmaceut Sci, Chuo Ku, Kumamoto 8620973, Japan

来源：

FEBS LETTERS | 2015年 / 589卷 / 19期

关键词：

Uracil-DNA glycosylase; Crystal structure; Leucine-intercalation loop; Sulfolobus tokodaii; BASE EXCISION-REPAIR; SPECIFICITY;

D O I：

10.1016/j.febslet.2015.08.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uracil-DNA glycosylases (UDGs) excise uracil from DNA by catalyzing the N-glycosidic bond hydrolysis. Here we report the first crystal structures of an archaeal UDG (stoUDG). Compared with other UDGs, stoUDG has a different structure of the leucine-intercalation loop, which is important for DNA binding. The stoUDG-DNA complex model indicated that Leu169, Tyr170, and Asn171 in the loop are involved in DNA intercalation. Mutational analysis showed that Tyr170 is critical for substrate DNA recognition. These results indicate that Tyr170 occupies the intercalation site formed after the structural change of the leucine-intercalation loop required for the catalysis. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2675 / 2682

页数：8

共 50 条

[11] A novel Family V uracil DNA glycosylase from Sulfolobus islandicus REY15A
Wu, Mai
Zhang, Likui
Dong, Kunming
Gong, Yong
Liu, Xipeng
DNA REPAIR, 2022, 120
[12] Structure determination of uracil-DNA N-glycosylase from Deinococcus radiodurans in complex with DNA
Pedersen, Hege Lynum
Johnson, Kenneth A.
Mcvey, Colin E.
Leiros, Ingar
Moe, Elin
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2015, 71 : 2137 - 2149
[13] New Family of Deamination Repair Enzymes in Uracil-DNA Glycosylase Superfamily
Lee, Hyun-Wook
Dominy, Brian N.
Cao, Weiguo
JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (36) : 31282 - 31287
[14] Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA
Parikh, SS
Mol, CD
Slupphaug, G
Bharati, S
Krokan, HE
Tainer, JA
EMBO JOURNAL, 1998, 17 (17) : 5214 - 5226
[15] Biochemical characterization and mechanistic insight of the family IV uracil DNA glycosylase from Sulfolobus islandicus REY15A
Wu, Mai
Lin, Tan
Dong, Kunming
Gong, Yong
Liu, Xipeng
Zhang, Likui
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 230
[16] Lessons learned from structural results on uracil-DNA glycosylase
Parikh, SS
Putnam, CD
Tainer, JA
MUTATION RESEARCH-DNA REPAIR, 2000, 460 (3-4): : 183 - 199
[17] Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands
G. V. Mechetin
E. A. Dyatlova
A. N. Sinyakov
V. A. Ryabinin
P. E. Vorobjev
D. O. Zharkov
Russian Journal of Bioorganic Chemistry, 2017, 43 : 23 - 28
[18] Crystal structure and functional insights into uracil-DNA glycosylase inhibition by phage φ29 DNA mimic protein p56
Ignacio Banos-Sanz, Jose
Mojardin, Laura
Sanz-Aparicio, Julia
Lazaro, Jose M.
Villar, Laurentino
Serrano-Heras, Gemma
Gonzalez, Beatriz
Salas, Margarita
NUCLEIC ACIDS RESEARCH, 2013, 41 (13) : 6761 - 6773
[19] Structure and function of the uracil DNA glycosylases from hyperthermophiles: Elucidating DNA uracil repair mechanisms: A review
Chen, Cai
Zheng, Yaqi
Gao, Tian
Chen, Min
Dong, Kunming
Shen, Li
Bai, Yanchao
Zhang, Likui
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2025, 299
[20] Integrating DNA structure switch with branched hairpins for the detection of uracil-DNA glycosylase activity and inhibitor screening
Zhe, Jing
Hao, Qijie
Liu, Yi
Guo, Zhaohui
Rustam, Buayxigul
Jiang, Wei
TALANTA, 2018, 179 : 51 - 56

← 1 2 3 4 5 →