A Promiscuous Intermediate Underlies the Evolution of LEAFY DNA Binding Specificity

被引：103

作者：

Sayou, Camille ^{[1
,2
,3
,4
]}

Monniaux, Marie ^{[1
,2
,3
,4
]}

Nanao, Max H. ^{[5
,6
]}

Moyroud, Edwige ^{[1
,2
,3
,4
]}

Brockington, Samuel F. ^{[7
]}

Thevenon, Emmanuel ^{[1
,2
,3
,4
]}

Chahtane, Hicham ^{[1
,2
,3
,4
]}

Warthmann, Norman ^{[8
]}

Melkonian, Michael ^{[9
]}

Zhang, Yong ^{[10
]}

Wong, Gane Ka-Shu ^{[10
,11
]}

Weigel, Detlef ^{[8
]}

Parcy, Francois ^{[1
,2
,3
,4
,12
]}

Dumas, Renaud ^{[1
,2
,3
,4
]}

机构：

[1] CNRS, LPCV, UMR 5168, F-38054 Grenoble, France

[2] Univ Grenoble Alpes, LPCV, F-38054 Grenoble, France

[3] Commissariat Energie Atom & Energies Alternat, Direct Sci Vivant, Inst Rech Technol & Sci Vivant, LPCV, F-38054 Grenoble, France

[4] Inst Natl Rech Agron, LPCV, F-38054 Grenoble, France

[5] EMBL, F-38042 Grenoble, France

[6] Univ Grenoble Alpes, Unit Virus Host Cell Interact, CNRS, EMBL,UMI 3265, F-38042 Grenoble 9, France

[7] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England

[8] Max Planck Inst Dev Biol, Dept Mol Biol, D-72076 Tubingen, Germany

[9] Univ Cologne, Biozentrum Koln, Inst Bot, Lehrstuhl 1, D-50674 Cologne, Germany

[10] Beijing Genom Inst BGI Shenzhen, Shenzhen 518083, Peoples R China

[11] Univ Alberta, Dept Med, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

[12] Univ British Columbia, Child & Family Res Inst, Ctr Mol Med & Therapeut, Vancouver, BC V5Z 4H4, Canada

来源：

SCIENCE | 2014年 / 343卷 / 6171期

关键词：

REGULATOR; DIVERSITY;

D O I：

10.1126/science.1248229

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transcription factors (TFs) are key players in evolution. Changes affecting their function can yield novel life forms but may also have deleterious effects. Consequently, gene duplication events that release one gene copy from selective pressure are thought to be the common mechanism by which TFs acquire new activities. Here, we show that LEAFY, a major regulator of flower development and cell division in land plants, underwent changes to its DNA binding specificity, even though plant genomes generally contain a single copy of the LEAFY gene. We examined how these changes occurred at the structural level and identify an intermediate LEAFY form in hornworts that appears to adopt all different specificities. This promiscuous intermediate could have smoothed the evolutionary transitions, thereby allowing LEAFY to evolve new binding specificities while remaining a single-copy gene.

引用

页码：645 / 648

页数：4

共 18 条

[1] The 'evolvability' of promiscuous protein functions [J].

Aharoni, A ;

Gaidukov, L ;

Khersonsky, O ;

Gould, SM ;

Roodveldt, C ;

Tawfik, DS .

NATURE GENETICS, 2005, 37 (01) :73-76

[2] Diversity and Complexity in DNA Recognition by Transcription Factors [J].

Badis, Gwenael ;

Berger, Michael F. ;

Philippakis, Anthony A. ;

Talukder, Shaheynoor ;

Gehrke, Andrew R. ;

Jaeger, Savina A. ;

Chan, Esther T. ;

Metzler, Genita ;

Vedenko, Anastasia ;

Chen, Xiaoyu ;

Kuznetsov, Hanna ;

Wang, Chi-Fong ;

Coburn, David ;

Newburger, Daniel E. ;

Morris, Quaid ;

Hughes, Timothy R. ;

Bulyk, Martha L. .

SCIENCE, 2009, 324 (5935) :1720-1723

[3] Following Gene Duplication, Paralog Interference Constrains Transcriptional Circuit Evolution [J].

Baker, Christopher R. ;

Hanson-Smith, Victor ;

Johnson, Alexander D. .

SCIENCE, 2013, 342 (6154) :104-108

[4] Extensive DNA-binding specificity divergence of a conserved transcription regulator [J].

Baker, Christopher R. ;

Tuch, Brian B. ;

Johnson, Alexander D. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (18) :7493-7498

[5] Variation in homeodomain DNA binding revealed by high-resolution analysis of sequence preferences [J].

Berger, Michael F. ;

Badis, Gwenael ;

Gehrke, Andrew R. ;

Talukder, Shaheynoor ;

Philippakis, Anthony A. ;

Pena-Castillo, Lourdes ;

Alleyne, Trevis M. ;

Mnaimneh, Sanie ;

Botvinnik, Olga B. ;

Chan, Esther T. ;

Khalid, Faiqua ;

Zhang, Wen ;

Newburger, Daniel ;

Jaeger, Savina A. ;

Morris, Quaid D. ;

Bulyk, Martha L. ;

Hughes, Timothy R. .

CELL, 2008, 133 (07) :1266-1276

[6] Endless forms: the evolution of gene regulation and morphological diversity [J].

Carroll, SB .

CELL, 2000, 101 (06) :577-580

[7] Evolution of Minimal Specificity and Promiscuity in Steroid Hormone Receptors [J].

Eick, Geeta N. ;

Colucci, Jennifer K. ;

Harms, Michael J. ;

Ortlund, Eric A. ;

Thornton, Joseph W. .

PLOS GENETICS, 2012, 8 (11)

[8] Structural basis for LEAFY floral switch function and similarity with helix-turn-helix proteins [J].

Hames, Cecile ;

Ptchelkine, Denis ;

Grimm, Clemens ;

Thevenon, Emmanuel ;

Moyroud, Edwige ;

Gerard, Francine ;

Martiel, Jean-Louis ;

Benlloch, Reyes ;

Parcy, Francois ;

Mueller, Christoph W. .

EMBO JOURNAL, 2008, 27 (19) :2628-2637

[9] The locus of evolution: Evo devo and the genetics of adaptation [J].

Hoekstra, Hopi E. ;

Coyne, Jerry A. .

EVOLUTION, 2007, 61 (05) :995-1016

[10] Enzyme promiscuity: evolutionary and mechanistic aspects [J].

Khersonsky, Olga ;

Roodveldt, Cintia ;

Tawfik, Dan S. .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2006, 10 (05) :498-508

← 1 2 →