Preparation of high purity monodisperse oligosaccharides derived from mannuronan by size-exclusion chromatography followed by semi-preparative high-performance anion-exchange chromatography with pulsed amperometric detection

被引：7

作者：

Ballance, Simon ^{[1
]}

Aarstad, Olav Andreas ^{[1
]}

Aachmann, Finn Lillelund ^{[1
]}

Skjak-Braek, Gudmund ^{[1
]}

Christensen, Bjorn E. ^{[1
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Biotechnol, NOBIPOL, NTNU, N-7491 Trondheim, Norway

来源：

CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 02期

关键词：

Alginate; Uronic acid; Fractionation; Purification; ACID;

D O I：

10.1016/j.carres.2008.10.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligosaccharides of ([4)-beta-D-ManpA-(->](n)) with a degree of polymerisation (DP) of 5, 10 and 15 were generated by partial acid hydrolysis of alginate mannuronan. These were subsequently purified by a combination of size-exclusion chromatography and semi-preparative high-performance anion-exchange chromatography with pulsed amperometric detection. The purity of the isolated oligosaccharides was greater than 96%. With automated operation of the chromatography system, milligram quantities can be generated over a period of a few days. Thus, our methodology now offers some significant advantages over earlier, including our own, protocols focused on uronic acid oligomers, where the final products are either not as pure or more starting material is needed to generate an equivalent yield of product. Removal of ammonium ions in collected fractions after size-exclusion chromatography and prior to freeze-drying was found to be essential to prevent the formation of imines and subsequent Maillard browning products. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：255 / 259

页数：5

共 13 条

[1] Application of high-performance anion-exchange chromatography with pulsed amperometric detection and statistical analysis to study oligosaccharide distributions -: a complementary method to investigate the structure and some properties of alginates [J].

Ballance, S ;

Holtan, S ;

Aarstad, OA ;

Sikorski, P ;

Skjåk-Bræk, G ;

Christensen, BE .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1093 (1-2) :59-68

[2] Determination of average degree of polymerisation and distribution of oligosaccharides in a partially acid-hydrolysed homopolysaccharide:: A comparison of four experimental methods applied to mannuronan [J].

Campa, C ;

Oust, A ;

Skjåk-Bræk, G ;

Paulsen, BS ;

Paoletti, S ;

Christensen, BE ;

Ballance, S .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1026 (1-2) :271-281

[3]

Dionex Corperation, 1998, 21 DION CORP, P1

[4]

HICKS KB, 2002, J CHROMATOGRAPHY LIB, V66, P529

[5] Thermal depolymerization of alginate in the solid state [J].

Holme, HK ;

Lindmo, K ;

Kristiansen, A ;

Smidsrod, O .

CARBOHYDRATE POLYMERS, 2003, 54 (04) :431-438

[6] Isolation of oligogalacturonic acids up to DP 20 by preparative high-performance anion-exchange chromatography and pulsed amperometric detection [J].

Hotchkiss, AT ;

Lecrinier, SL ;

Hicks, KB .

CARBOHYDRATE RESEARCH, 2001, 334 (02) :135-140

[7]

Keller R, 2004, COMPUTER AIDED RESON, P73

[8]

KLUG ED, 1940, Patent No. 2197540

[9] APPLICATION OF PHOTOELECTRON-SPECTROSCOPY TO MOLECULAR-PROPERTIES .40. SYNTHESIS OF PARA-CHLOROPHOSPHAETHENE AND NORMAL-CHLOROMETHANIMINE - ESTIMATION OF CL SUBSTITUTION ON THE ELECTRONIC-STRUCTURE OF HETEROATOMIC DOUBLE-BONDS [J].

LACOMBE, S ;

PELLERIN, B ;

GUILLEMIN, JC ;

DENIS, JM ;

PFISTERGUILLOUZO, G .

JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (25) :5958-5963

[10]

MCMURRY J, 1988, ORGANIC CHEM, P1241

← 1 2 →