The interaction of SiO2 nanoparticles with the neuronal cell membrane: activation of ionic channels and calcium influx

被引：9

作者：

Distasi, Carla ^{[1
]}

Dionisi, Marianna ^{[1
]}

Ruffinatti, Federico Alessandro ^{[1
]}

Gilardino, Alessandra ^{[2
]}

Bardini, Roberta ^{[2
,3
]}

Antoniotti, Susanna ^{[2
]}

Catalano, Federico ^{[4
,5
]}

Bassino, Eleonora ^{[2
]}

Munaron, Luca ^{[2
]}

Martra, Gianmario ^{[3
,6
]}

Lovisolo, Davide ^{[2
]}

机构：

[1] Univ Piemonte Orientale, Dept Pharmaceut Sci, Via Bovio 6, I-28100 Novara, Italy

[2] Univ Torino, Dept Life Sci & Syst Biol, Via Accademia Albertina 23, I-10123 Turin, Italy

[3] Polytech Univ Turin, Dept Control & Comp Engn, Corso Duca Abruzzi 24, I-10129 Turin, Italy

[4] Univ Torino, Dept Chem, Via P Giuria 9, I-10125 Turin, Italy

[5] Italian Inst Technol, Cent Res Labs, Via Morego 30, I-16163 Genoa, Italy

[6] Univ Torino, NIS Interdept Ctr, Turin, Italy

来源：

NANOMEDICINE | 2019年 / 14卷 / 05期

关键词：

calcium; Cx channels; free radicals; neuronal cells; Panx channels; patch clamp; silica nanoparticles; single channel; TRPV4; channels; ATP RELEASE; TRPV4; CHANNELS; CA2+ INFLUX; HEMICHANNELS; INHIBITION; CONNEXIN; PANNEXIN-1; MECHANISMS; NEUROTOXICITY; EXPRESSION;

D O I：

10.2217/nnm-2018-0256

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+](i) increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

引用

页码：575 / 594

页数：20

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