The interaction of SiO2 nanoparticles with the neuronal cell membrane: activation of ionic channels and calcium influx

被引：8
|
作者：
Distasi, Carla [1 ]
Dionisi, Marianna [1 ]
Ruffinatti, Federico Alessandro [1 ]
Gilardino, Alessandra [2 ]
Bardini, Roberta [2 ,3 ]
Antoniotti, Susanna [2 ]
Catalano, Federico [4 ,5 ]
Bassino, Eleonora [2 ]
Munaron, Luca [2 ]
Martra, Gianmario [3 ,6 ]
Lovisolo, Davide [2 ]
机构：
[1] Univ Piemonte Orientale, Dept Pharmaceut Sci, Via Bovio 6, I-28100 Novara, Italy
[2] Univ Torino, Dept Life Sci & Syst Biol, Via Accademia Albertina 23, I-10123 Turin, Italy
[3] Polytech Univ Turin, Dept Control & Comp Engn, Corso Duca Abruzzi 24, I-10129 Turin, Italy
[4] Univ Torino, Dept Chem, Via P Giuria 9, I-10125 Turin, Italy
[5] Italian Inst Technol, Cent Res Labs, Via Morego 30, I-16163 Genoa, Italy
[6] Univ Torino, NIS Interdept Ctr, Turin, Italy
来源：
NANOMEDICINE | 2019年 / 14卷 / 05期
关键词：
calcium; Cx channels; free radicals; neuronal cells; Panx channels; patch clamp; silica nanoparticles; single channel; TRPV4; channels; ATP RELEASE; TRPV4; CHANNELS; CA2+ INFLUX; HEMICHANNELS; INHIBITION; CONNEXIN; PANNEXIN-1; MECHANISMS; NEUROTOXICITY; EXPRESSION;
D O I：
10.2217/nnm-2018-0256
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Materials & methods: Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. Results: TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+](i) increase. Conclusion: These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.
引用
收藏
页码：575 / 594
页数：20
相关论文
共 24 条
  • [1] Interaction of SiO2 nanoparticles with neuronal cells: Ionic mechanisms involved in the perturbation of calcium homeostasis
    Gilardino, Alessandra
    Catalano, Federico
    Ruffinatti, Federico Alessandro
    Alberto, Gabriele
    Nilius, Bernd
    Antoniotti, Susanna
    Martra, Gianmario
    Lovisolo, Davide
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2015, 66 : 101 - 111
  • [2] Effects of SiO2 nanoparticles on phospholipid membrane integrity and fluidity
    Wei, Xiaoran
    Jiang, Wei
    Yu, Junchao
    Ding, Lei
    Hu, Jingtian
    Jiang, Guibin
    JOURNAL OF HAZARDOUS MATERIALS, 2015, 287 : 217 - 224
  • [3] Calcium influx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch
    Hara, Minako
    Tabata, Kuniko
    Suzuki, Takahiro
    Do, Mai-Khoi Q.
    Mizunoya, Wataru
    Nakamura, Mako
    Nishimura, Shotaro
    Tabata, Shoji
    Ikeuchi, Yoshihide
    Sunagawa, Kenji
    Anderson, Judy E.
    Allen, Ronald E.
    Tatsumi, Ryuichi
    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2012, 302 (12): : C1741 - C1750
  • [4] Mechanistic study of the adsorption and penetration of modified SiO2 nanoparticles on cellular membrane
    Yuan, Shideng
    Wei, Yaoyao
    Wang, Xueyu
    Liu, Shasha
    Zhang, Heng
    Yuan, Shiling
    CHEMOSPHERE, 2022, 294
  • [5] The effect of chemical modification of SiO2 nanoparticles on the nanofiltration characteristics of polyamide membrane
    Rakhshan, Nasim
    Pakizeh, Majid
    KOREAN JOURNAL OF CHEMICAL ENGINEERING, 2015, 32 (12) : 2524 - 2533
  • [6] Role of bovine serum albumin and humic acid in the interaction between SiO2 nanoparticles and model cell membranes
    Wei, Xiaoran
    Qu, Xiaolei
    Ding, Lei
    Hu, Jingtian
    Jiang, Wei
    ENVIRONMENTAL POLLUTION, 2016, 219 : 1 - 8
  • [7] SiO2 Nanoparticles Alter Basal Intracellular Calcium Concentration by Reducing TRPC1 Channel Expression in Human Retinal Pigment Epithelium Cell Line
    Kaynar, A. H.
    Soeguet, F.
    Yilmaz, S. N.
    Yildirimcan, S.
    Coemelekoglu, Ue.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2024, 50 (05) : 1991 - 1997
  • [8] Molecular insights into the uptake of SiO2 nanoparticles on phospholipid membrane: Effect of surface properties and particle size
    Yuan, Shideng
    Zhang, Heng
    Wang, Xueyu
    Zhang, Hengming
    Zhang, Zhenyu
    Yuan, Shiling
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2022, 210
  • [9] Ca2+ influx through glutamate receptor-associated channels in retina cells correlates with neuronal cell death
    Ferreira, IL
    Duarte, CB
    Carvalho, AP
    EUROPEAN JOURNAL OF PHARMACOLOGY, 1996, 302 (1-3) : 153 - 162
  • [10] In-situ sol-gel generation of SiO2 nanoparticles inside polyamide membrane for enhanced nanofiltration
    Li, Jian
    Cheng, Lilantian
    Song, Weilong
    Xu, Yilin
    Liu, Fei
    Wang, Zhenyu
    DESALINATION, 2022, 540
123