Porcine reproductive and respiratory syndrome virus ORF5a protein is essential for virus viability

被引：28

作者：

Sun, Lichang ^{[1
,2
]}

Li, Yanhua ^{[2
,3
]}

Liu, Runxia ^{[2
]}

Wang, Xiaomin ^{[4
]}

Gao, Fei ^{[2
]}

Lin, Tao ^{[1
]}

Huang, Ting ^{[1
]}

Yao, Huochun ^{[1
]}

Tong, Guangzhi ^{[2
]}

Fan, Hongjie ^{[1
]}

Wei, Zuzhang ^{[2
]}

Yuan, Shishan ^{[2
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, Dept Swine Infect Dis, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

[3] S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA

[4] Natl Ctr Engn Res Vet Bioprod, Key Lab Anim Dis Diagnost & Immunol, Minist Agr, Inst Vet Med,Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

来源：

VIRUS RESEARCH | 2013年 / 171卷 / 01期

关键词：

Porcine reproductive and respiratory syndrome virus; Codon optimization; ORF5a; Viability; N-LINKED GLYCOSYLATION; ENVELOPE PROTEINS; NUCLEOCAPSID PROTEIN; STRUCTURAL PROTEINS; CODING REGIONS; CELL-CULTURE; IDENTIFICATION; TRANSCRIPTION; REPLICATION; ARTERIVIRUSES;

D O I：

10.1016/j.virusres.2012.11.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It has been shown that ORF5a protein in EAV is important but not essential for virus infectivity. In this study, we found that RNA changes in the overlapping region (1-104 nucleotide, nt) between ORF5 and ORF5a introduced by codon-optimized GP5 was lethal for virus viability, suggesting that the nt changes or amino acid (aa) mutations in the GP5 or ORF5a protein did not permit the production of infectious virus. Furthermore, inactivation of ORF5a expression in the context of type 1 (pSHE) and type 2 (pAJXM and pAPRRS) full-length PRRSV cDNA clones was lethal for the production of infectious virus, while viable PRRSV could be recovered by expressing ORF5a protein in trans, suggesting that ORF5a protein was essential for virus viability. Finally, ORF5a protein could be putatively extended to 63 aas by inactivation of the downstream stop codon candidates, thereby demonstrating that the C-terminus of ORF5a may be variable. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：178 / 185

页数：8

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