Postnatal epithelial growth of the small intestine in the rat occurs by both crypt fission and crypt hyperplasia

被引：35
|
作者：
Cummins, AG [1 ]
Jones, BJ [1 ]
Thompson, FM [1 ]
机构：
[1] Queen Elizabeth Hosp, Dept Gastroenterol & Hepatol, Woodville, SA 5011, Australia
来源：
DIGESTIVE DISEASES AND SCIENCES | 2006年 / 51卷 / 04期
关键词：
crypt fission; crypt hyperplasia; growth of the small intestine; intestinal stem cells;
D O I：
10.1007/s10620-006-3197-9
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Studies of growth of the small intestine have largely concentrated on crypt hyperplasia rather than crypt fission. The aim of this study was to investigate quantitatively both crypt fission and crypt hyperplasia. DAxPVG/c rats were killed at 7, 11, 14, 17, 19, 21, 25, 55, and 72-73 days of life. Samples of jejunum at one third of the intestinal length were taken for morphometry (villous area, crypt area, percentage of bifid crypts, and crypt mitotic count) by microdissection. Growth factors and their receptors were assessed by oligonucleotide microarray. Crypt fission was 10.5%, 5.2%, and 1.5% at days 11, 25, and 72-73 of life, respectively. Crypt hyperplasia increased from day 21. No conventional growth factor was identified during crypt fission. We conclude that crypt fission contributes to growth of the small intestine prior to weaning and crypt hyperplasia to growth after weaning.
引用
收藏
页码：718 / 723
页数：6
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