Semi-interpenetrating network (sIPN) gelatin nanofiber scaffolds for oral mucosal drug delivery

被引:72
作者
Aduba, Donald C., Jr. [1 ]
Hammer, Jeremy A. [2 ,3 ,4 ]
Yuan, Quan [1 ]
Yeudall, W. Andrew [5 ,6 ]
Bowlin, Gary L. [1 ]
Yang, Hu [1 ,6 ]
机构
[1] Virginia Commonwealth Univ, Dept Biomed Engn, Richmond, VA 23284 USA
[2] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA
[3] Univ Utah, Dept Bioengn, Salt Lake City, UT 84112 USA
[4] Univ Utah, Utah Ctr Nanomed, Salt Lake City, UT 84112 USA
[5] Virginia Commonwealth Univ, Philips Inst Oral & Craniofacial Mol Biol, Richmond, VA 23298 USA
[6] Virginia Commonwealth Univ, Massey Canc Ctr, Richmond, VA 23298 USA
基金
美国国家科学基金会;
关键词
Drug delivery; Electrospinning; Nanofiber; Tissue engineering; Buccal mucosa; COLLAGEN; ELASTIN; POLYMER; DESIGN; CAVITY; SITE;
D O I
10.1016/j.actbio.2013.02.006
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The oral mucosa is a promising absorption site for drug administration because it is permeable, highly vascularized and allows for ease of administration. Nanofiber scaffolds for local or systemic drug delivery through the oral mucosa, however, have not been fully explored. In this work, we fabricated electrospun gelatin nanofiber scaffolds for oral mucosal drug delivery. To improve structural stability of the electrospun gelatin scaffolds and allow non-invasive incorporation of therapeutics into the scaffold, we employed photo-reactive polyethylene glycol diacrylate (PEG-DA575, 575 gmol(-1)) as a cross-linker to stabilize the scaffold by forming semi-interpenetrating network gelatin nanofiber scaffolds (sIPN NSs), during which cross-linker concentration was varied (1 x, 2 x, 4x and 8 x). The results showed that electrospun gelatin nanofiber scaffolds after being cross-linked with PEG-DA575 (i.e. sIPN NS1 x, 2x, 4x and 8 x) retained fiber morphology and possessed improved structural stability. A series of structural parameters and properties of the cross-linked electrospun gelatin scaffolds were systematically characterized in terms of morphology, fiber diameter, mechanical properties, porosity, swelling and degradation. Mucin absorption onto sIPN NS4x was also confirmed, indicating this scaffold possessed greatest mucoadhesion properties among those tested. Slow release of nystatin, an anti-fungal reagent, from the sIPN gelatin nanofiber scaffold was demonstrated. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:6576 / 6584
页数:9
相关论文
共 34 条
[1]   Nanofiber technology: Designing the next generation of tissue engineering scaffolds [J].
Barnes, Catherine P. ;
Sell, Scott A. ;
Boland, Eugene D. ;
Simpson, David G. ;
Bowlin, Gary L. .
ADVANCED DRUG DELIVERY REVIEWS, 2007, 59 (14) :1413-1433
[2]   Cross-linking electrospun type II collagen tissue engineering scaffolds with carbodiimide in ethanol [J].
Barnes, Catherine P. ;
Pemble, Charles W. ;
Brand, David D. ;
Simpson, David G. ;
Bowlin, Gary L. .
TISSUE ENGINEERING, 2007, 13 (07) :1593-1605
[3]   Electrospinning collagen and elastin: Preliminary vascular tissue engineering [J].
Boland, ED ;
Matthews, JA ;
Pawlowski, KJ ;
Simpson, DG ;
Wnek, GE ;
Bowlin, GL .
FRONTIERS IN BIOSCIENCE-LANDMARK, 2004, 9 :1422-1432
[4]   Synthesis and physicochemical analysis of interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate [J].
Burmania, JA ;
Martinez-Diaz, GJ ;
Kao, WJ .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 67A (01) :224-234
[5]   Bioadhesive Films Containing Benzocaine: Correlation Between In Vitro Permeation and In Vivo Local Anesthetic Effect [J].
de Araujo, Daniele Ribeiro ;
Padula, Cristina ;
Saia Cereda, Cintia Maria ;
Tofoli, Giovana Radomille ;
Brito, Rui Barbosa, Jr. ;
de Paula, Eneida ;
Nicoli, Sara ;
Santi, Patrizia .
PHARMACEUTICAL RESEARCH, 2010, 27 (08) :1677-1686
[6]  
DEVRIES ME, 1991, CRIT REV THER DRUG, V8, P271
[7]  
EPSTEIN JB, 1984, REV INFECT DIS, V6, P96
[8]   Drug Release Kinetics and Transport Mechanisms from Semi-interpenetrating Networks of Gelatin and Poly(ethylene glycol) diacrylate [J].
Fu, Yao ;
Kao, Weiyuan John .
PHARMACEUTICAL RESEARCH, 2009, 26 (09) :2115-2124
[9]   ORAL CAVITY AS A SITE FOR BIOADHESIVE DRUG-DELIVERY [J].
GANDHI, RB ;
ROBINSON, JR .
ADVANCED DRUG DELIVERY REVIEWS, 1994, 13 (1-2) :43-74
[10]   High-performance liquid chromatographic determination of liposomal nystatin in plasma and tissues for pharmacokinetic and tissue distribution studies [J].
Groll, AH ;
Mickiene, D ;
Werner, K ;
Piscitelli, SC ;
Walsh, TJ .
JOURNAL OF CHROMATOGRAPHY B, 1999, 735 (01) :51-62