Effect of Horse-chestnut seed extract on matrix metalloproteinase-1 and-9 during diabetic wound healing

被引：14

作者：

Aksoy, Halil ^{[1
]}

Cevik, Ozge ^{[2
]}

Sen, Ali ^{[3
]}

Goger, Fatih ^{[4
]}

Sekerler, Turgut ^{[1
]}

Sener, Azize ^{[1
]}

机构：

[1] Marmara Univ, Dept Biochem, Fac Pharm, Tibbiye Cad 49, TR-34668 Istanbul, Turkey

[2] Adnan Menderes Univ, Dept Biochem, Fac Med, Aydin, Turkey

[3] Marmara Univ, Dept Pharmacognosy, Fac Pharm, Istanbul, Turkey

[4] Anadolu Univ, Dept Pharmacognosy, Fac Pharm, Eskisehir, Turkey

来源：

JOURNAL OF FOOD BIOCHEMISTRY | 2019年 / 43卷 / 03期

关键词：

diabetic wound; Horse chestnut seed extract; LC-MS/MS; matrix metalloproteinase-1; matrix metalloproteinase-9; wound healing; AESCULUS-HIPPOCASTANUM L; PHENOLIC-COMPOUNDS; OXIDATIVE STRESS; LEAF TISSUES; ANTIOXIDANT; EXPRESSION; QUERCETIN; EFFICACY; INHIBITOR; AESCIN;

D O I：

10.1111/jfbc.12758

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of aqueous-ethanol extract of Horse chestnut (HCE) on MMP-1 and MMP-9 expressions during cutaneous wound healing in diabetic rats were investigated in this study. The expressions of MMP-1 and MMP-9, wound closure, myeloperoxidase (MPO) activity, hydroxyproline, and malondialdehyde (MDA) levels in wound tissue were measured. Quercetin glucuronide in HCE was identified as main compound using a LC-MS/MS. The hydroxyproline level was significantly increased in the treated group versus control after the 3rd and 7th days (p < 0.05). The MDA level and MPO activity were significantly lower in the treatment group (p < 0.05). MMP-1 gene expression level in treated rats was increased in the 7th day while it was reduced in 14th day. MMP-9 gene expression level in treated rats was decreased in 7th, and 14th days compared to control (p < 0.05). These results show that HCE accelerated the cutaneous wound-healing process in diabetic rats via MMP-1 and MMP-9 regulation.

引用

页数：9

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