Characterization of vasoactive intestinal peptide receptors on human megakaryocytes and platelets

被引：21

作者：

Park, SK ^{[1
]}

Olson, TA ^{[1
]}

Ercal, N ^{[1
]}

Summers, M ^{[1
]}

ODorisio, MS ^{[1
]}

机构：

[1] OHIO STATE UNIV, DEPT PEDIAT, COLUMBUS, OH 43210 USA

来源：

BLOOD | 1996年 / 87卷 / 11期

关键词：

D O I：

10.1182/blood.V87.11.4629.bloodjournal87114629

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Vasoactive intestinal peptide receptor I (VIPRI) expression was examined in megakaryocytes using reverse transcriptase-polymerase chain reaction (RT-PCR). VIPRI protein was characterized in platelet membranes using covalent crosslinking techniques. Human megakaryocytes were isolated from suspension cultures of cord blood and adult bone marrow mononuclear cells using a murine monoclonal antibody to human platelet glycoprotein IIB/IIIA (CD41) and immunomagnetic beads. RT-PCR primers were constructed for the VIP, VIPRI, and VIPRII genes as well as for megakaryocyte specific genes, c-mpl and platelet factor 4 (PF-4). VIP, VIPRI, c-mpl, and PF-4 were coexpressed in megakaryocyte mRNA. Southern blot analysis confirmed the expression of VIPRII. I-125-VIP was covalently cross-linked to human platelet membranes using the homobifunctional reagent disuccinimidyl suberate, followed by polyacrylamide gel electrophoresis and autoradiography. A I-125-VIP-protein complex of M(r) = 50,000 was identified. Labeling of the M(r) = 50.000 component was completely abolished by unlabeled VIP, but not by peptide histidine methionine or growth hormone releasing factor, indicating specific binding of VIP to the platelet membranes. Taken together, these results suggest that VIP may have direct effects on megakaryocytopoiesis and support our earlier observations of VIP modulation of platelet aggregation. (C) 1996 by The American Society of Hematology.

引用

页码：4629 / 4635

页数：7

共 43 条

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