The roles of plant dsRNA-binding proteins in RNAi-like pathways

被引：84
作者：
Curtin, Shaun J. [1 ,2 ]
Watson, John M. [1 ]
Smith, Neil A. [1 ]
Eamens, Andrew L. [1 ]
Blanchard, Chris L. [2 ]
Waterhouse, Peter M. [1 ,3 ]
机构：
[1] CSIRO Plant Ind Canberra, Canberra, ACT 2601, Australia
[2] Charles Sturt Univ, Wagga Wagga, NSW 2678, Australia
[3] Univ Sydney, Sydney, NSW 2006, Australia
来源：
FEBS LETTERS | 2008年 / 582卷 / 18期
关键词：
double-stranded RNA; RNA binding protein; RNA interference; gene silencing pathway;
D O I：
10.1016/j.febslet.2008.07.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Dicers are associated with double-stranded RNA-binding proteins ( dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi) RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2753 / 2760
页数：8
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