Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

被引：62

作者：

Pache, Christophe ^{[1
]}

Bocchio, Noelia L. ^{[1
]}

Bouwens, Arno ^{[1
]}

Villiger, Martin ^{[1
]}

Berclaz, Corinne ^{[1
]}

Goulley, Joan ^{[2
]}

Gibson, Matthew I. ^{[3
]}

Santschi, Christian ^{[4
]}

Lasser, Theo ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Opt Biomed, Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, ISREC, Lausanne, Switzerland

[3] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

[4] Ecole Polytech Fed Lausanne, Nanophoton & Metrol Lab, Lausanne, Switzerland

来源：

OPTICS EXPRESS | 2012年 / 20卷 / 19期

基金：

瑞士国家科学基金会;

关键词：

LIVE CELLS; TOMOGRAPHY; LIGHT; SCATTERING; DYNAMICS; DEPTH; BEAMS; DYES;

D O I：

10.1364/OE.20.021385

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed inherent to OCM. We report on the detection of single 40 nm gold particles with a 0.5 mu m lateral and 2 mu m axial resolution over a 50 mu m depth of field and the three-dimensional localization of gold colloids within living cells. In combination with intrinsic sample contrast measured with dark-field OCM, poli-OCM offers a versatile platform for functional cell imaging. (C) 2012 Optical Society of America

引用

页码：21385 / 21399

页数：15

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