A mitochondria-targeted red-emitting probe for imaging hydrogen sulfide in living cells and zebrafish

被引：25
|
作者：
Ismail, Ismail [1 ,2 ]
Wang, Dan [1 ,2 ]
Wang, Dawei [1 ,2 ]
Niu, Cuili [1 ,2 ]
Huang, Haojie [3 ,4 ]
Yi, Long [3 ,4 ,5 ]
Xi, Zhen [1 ,2 ,5 ]
机构：
[1] Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China
[2] Nankai Univ, Dept Biol Chem, Natl Engn Res Ctr Pesticide Tianjin, Coll Chem, Tianjin 300071, Peoples R China
[3] BUCT, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China
[4] BUCT, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China
[5] Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2019年 / 17卷 / 13期
基金：
国家重点研发计划;
关键词：
RATIOMETRIC FLUORESCENT-PROBE; SENSITIVE DETECTION; RAPID DETECTION; H2S SYNTHESIS; CANCER-CELLS; THIOLYSIS; REDUCTION; SPECTRA; DESIGN;
D O I：
10.1039/c8ob03219j
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Hydrogen sulfide (H2S) is an important endogenous signaling molecule with multiple biological functions. Based on the thiolysis of NBD (7-nitro-1,2,3-benzoxadiazole) amine, herein we rationally design and synthesize a new fluorescent probe 1 for the detection of mitochondrial H2S in living cells. Probe 1 displays bright red-emitting fluorescence after H2S activation (., 0.77). 1 shows higher selectivity and sensitivity for H2S than the red-emitting probe Rho-NBD (ChemBioChem, 2016, 17, 962). Moreover, 1 is water-soluble, cell-membrane-permeable, of low cytotoxicity and mitochondria-targeting, and can be employed for monitoring mitochondrial H2S in living cells.
引用
收藏
页码：3389 / 3395
页数：7
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