Determination of Percent Hemoglobin A1c Using a Potentiometric Method

被引：31
作者：
Liu, Hong [1 ]
Crooks, Richard M. [1 ]
机构：
[1] Univ Texas Austin, Dept Chem & Biochem, Austin, TX 78712 USA
来源：
ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 03期
关键词：
ALIZARIN RED S; GLYCATED HEMOGLOBIN; DIABETES-MELLITUS; ASSAY; GLYCOHEMOGLOBIN; CHROMATOGRAPHY; BLOOD; A(1C);
D O I：
10.1021/ac3032228
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We report a potentiometric method for measuring the hemoglobin A1c (HbA1c, glycated hemoglobin) concentration, hemoglobin (Hb) concentration, and percent HbA1c (%HbAlc) in human blood hemolysate. The %HbAlc is important for diagnosis and management of diabetes mellitus. Alizarin red s (ARS) is used as a redox indicator. Phenylboronic acid (PBA) binds to both ABS and HbA1c via diol boronic acid complexation. The binding of PBA to ARS shifts its redox potential negatively. However, when HbA1c competes with ARS for PBA binding, the solution potential shifts positively. This shift is linked to the HbA1c concentration. The concentration of Hb is determined by allowing it to react with Fe(CN)(6)(3-). The potential shift arising from the reduction of Fe(CN)(6)(3-) by Hb is proportional to the logarithm of the Hb concentration. The results obtained for %HbAlc in human blood hemolysate are in good agreement with those determined using a reference method.
引用
收藏
页码：1834 / 1839
页数：6
相关论文
共 34 条
[1]   Chemiluminescent acridinium-9-carboxamide boronic acid probes: Application to a homogenous glycated hemoglobin assay [J].
Adamczyk, M ;
Chen, YY ;
Johnson, DD ;
Mattingly, PG ;
Moore, JA ;
Pan, Y ;
Reddy, RE .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2006, 16 (05) :1324-1328
[2]  
[Anonymous], 2001, ELECTROCHEMICAL METH
[3]  
Blincko S, 1998, CLIN CHEM, V44, P1302
[4]   Two-channel microelectrochemical bipolar electrode sensor array [J].
Chang, Byoung-Yong ;
Chow, Kwok-Fan ;
Crooks, John A. ;
Mavre, Francois ;
Crooks, Richard M. .
ANALYST, 2012, 137 (12) :2827-2833
[5]   The worldwide epidemiology of type 2 diabetes mellitus-present and future perspectives [J].
Chen, Lei ;
Magliano, Dianna J. ;
Zimmet, Paul Z. .
NATURE REVIEWS ENDOCRINOLOGY, 2012, 8 (04) :228-236
[6]   IMPROVED METHOD FOR ANALYSIS OF GLYCATED HEMOGLOBIN BY ION-EXCHANGE CHROMATOGRAPHY [J].
ECKERBOM, S ;
BERGQVIST, Y ;
JEPPSSON, JO .
ANNALS OF CLINICAL BIOCHEMISTRY, 1994, 31 :355-360
[7]   Measurement of Hemoglobin A1c from Filter Papers for Population-Based Studies [J].
Egier, David A. ;
Keys, Judy L. ;
Hall, S. Kim ;
McQueen, Matthew J. .
CLINICAL CHEMISTRY, 2011, 57 (04) :577-585
[8]  
Frantzen F, 1997, CLIN CHEM, V43, P2390
[9]   Soluble highly coloured phenylboronic acids and their use in glycohemoglobin quantification [J].
Frantzen, F ;
Grimsrud, K ;
Heggli, DE ;
Sundrehagen, E .
CLINICA CHIMICA ACTA, 1997, 263 (02) :207-224
[10]   Electrochemical glucose sensors and their applications in diabetes management [J].
Heller, Adam ;
Feldman, Ben .
CHEMICAL REVIEWS, 2008, 108 (07) :2482-2505
1234