Biosynthesis of silver nanoparticles using Plectranthus amboinicus leaf extract and its antimicrobial activity

被引:85
作者
Ajitha, B. [1 ]
Reddy, Y. Ashok Kumar [2 ]
Reddy, P. Sreedhara [1 ]
机构
[1] Sri Venkateswara Univ, Dept Phys, Tirupati 517502, Andhra Pradesh, India
[2] Korea Adv Inst Sci & Technol, Dept Elect Engn, Taejon 305701, South Korea
关键词
Green synthesis method; Silver nanoparticles; Leaf extract; Particle size; Absorbance; Antimicrobial activity; METAL NANOPARTICLES; VIBRATIONAL-SPECTRA; GREEN SYNTHESIS; PHOTOLUMINESCENCE; SPECTROSCOPY; COLLOIDS;
D O I
10.1016/j.saa.2014.02.105
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
This study reports the simple green synthesis method for the preparation of silver nanoparticles (Ag NPs) using Plectranthus amboinicus leaf extract. The pathway of nanoparticles formation is by means of reduction of AgNO3 by leaf extract, which acts as both reducing and capping agents. Synthesized Ag NPs were subjected to different characterizations for studying the structural, chemical, morphological, optical and antimicrobial properties. The bright circular fringes in SAED pattern and diffraction peaks in XRD profile reveals high crystalline nature of biosynthesized Ag NPs. Morphological studies shows the formation of nearly spherical nanoparticles. FTIR spectrum confirms the existence of various functional groups of biomolecules capping the nanoparticles. UV visible spectrum displays single SPR band at 428 nm indicating the absence of anisotropic particles. The synthesized Ag NPs exhibited better antimicrobial property towards gram negative Escherichia coli and towards tested Penicillium spp. than other tested microorganisms using disc diffusion method. Finally it has proven that the synthesized bio-inspired Ag NPs have potent antimicrobial effect. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:257 / 262
页数:6
相关论文
共 38 条
[21]   Structure and vibrational spectra of mononitrated benzo[a]pyrenes [J].
Onchoke, KK ;
Hadad, CM ;
Dutta, PK .
JOURNAL OF PHYSICAL CHEMISTRY A, 2006, 110 (01) :76-84
[22]   Silver colloid nanoparticles:: Synthesis, characterization, and their antibacterial activity [J].
Panacek, Ales ;
Kvitek, Libor ;
Prucek, Robert ;
Kolar, Milan ;
Vecerova, Renata ;
Pizurova, Nadezda ;
Sharma, Virender K. ;
Nevecna, Tat'jana ;
Zboril, Radek .
JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (33) :16248-16253
[23]   Stable silver colloidal dispersions using short chain polyethylene glycol [J].
Popa, Monica ;
Pradell, Trinitat ;
Crespo, Daniel ;
Calderon-Moreno, Jose M. .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2007, 303 (03) :184-190
[24]   Size control over spherical silver nanoparticles by ascorbic acid reduction [J].
Qin, Yaqiong ;
Ji, Xiaohui ;
Jing, Jing ;
Liu, Hong ;
Wu, Hongli ;
Yang, Wensheng .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2010, 372 (1-3) :172-176
[25]   Silver nanoparticles as a new generation of antimicrobials [J].
Rai, Mahendra ;
Yadav, Alka ;
Gade, Aniket .
BIOTECHNOLOGY ADVANCES, 2009, 27 (01) :76-83
[26]  
Rozo J.I. Jerez, 2007, P SOC PHOTO-OPT INS, V6538
[27]   Silver bromide nanoparticle/polymer composites: Dual action tunable antimicrobial materials [J].
Sambhy, Varun ;
MacBride, Megan M. ;
Peterson, Blake R. ;
Sen, Ayusman .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (30) :9798-9808
[28]   Antibiotics for treatment of infections caused by MRSA and elimination of MRSA carriage. What are the choices? [J].
Schmitz, FJ ;
Jones, ME .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 1997, 9 (01) :1-19
[29]   Silver nanoparticles: Green synthesis and their antimicrobial activities [J].
Sharma, Virender K. ;
Yngard, Ria A. ;
Lin, Yekaterina .
ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2009, 145 (1-2) :83-96
[30]   Phytosynthesis of Au, Ag and Au-Ag bimetallic nanoparticles using aqueous extract and dried leaf of Anacardium occidentale [J].
Sheny, D. S. ;
Mathew, Joseph ;
Philip, Daizy .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2011, 79 (01) :254-262