Biosynthesis of silver nanoparticles using Plectranthus amboinicus leaf extract and its antimicrobial activity

被引:85
作者
Ajitha, B. [1 ]
Reddy, Y. Ashok Kumar [2 ]
Reddy, P. Sreedhara [1 ]
机构
[1] Sri Venkateswara Univ, Dept Phys, Tirupati 517502, Andhra Pradesh, India
[2] Korea Adv Inst Sci & Technol, Dept Elect Engn, Taejon 305701, South Korea
关键词
Green synthesis method; Silver nanoparticles; Leaf extract; Particle size; Absorbance; Antimicrobial activity; METAL NANOPARTICLES; VIBRATIONAL-SPECTRA; GREEN SYNTHESIS; PHOTOLUMINESCENCE; SPECTROSCOPY; COLLOIDS;
D O I
10.1016/j.saa.2014.02.105
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
This study reports the simple green synthesis method for the preparation of silver nanoparticles (Ag NPs) using Plectranthus amboinicus leaf extract. The pathway of nanoparticles formation is by means of reduction of AgNO3 by leaf extract, which acts as both reducing and capping agents. Synthesized Ag NPs were subjected to different characterizations for studying the structural, chemical, morphological, optical and antimicrobial properties. The bright circular fringes in SAED pattern and diffraction peaks in XRD profile reveals high crystalline nature of biosynthesized Ag NPs. Morphological studies shows the formation of nearly spherical nanoparticles. FTIR spectrum confirms the existence of various functional groups of biomolecules capping the nanoparticles. UV visible spectrum displays single SPR band at 428 nm indicating the absence of anisotropic particles. The synthesized Ag NPs exhibited better antimicrobial property towards gram negative Escherichia coli and towards tested Penicillium spp. than other tested microorganisms using disc diffusion method. Finally it has proven that the synthesized bio-inspired Ag NPs have potent antimicrobial effect. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:257 / 262
页数:6
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