Knockdown of Pokemon Protein Expression Inhibits Hepatocellular Carcinoma Cell Proliferation by Suppression of AKT Activity

被引:11
作者
Zhu, Xiaosan [1 ,2 ,3 ]
Dai, Yichen [1 ,2 ]
Chen, Zhangxin [1 ,2 ]
Xie, Junpei [1 ,2 ]
Zeng, Wei [1 ,2 ]
Lin, Yuanyuan [1 ,2 ]
机构
[1] Xiamen Univ, Chenggong Hosp, Dept Gastroenterol, Xiamen, Fujian, Peoples R China
[2] 174th Hosp PLA, Xiamen 361003, Fujian, Peoples R China
[3] Nanchang Univ, Dept Med, Grad Coll, Nanchang, Jiangxi, Peoples R China
关键词
Pokemon; HepG2; cells; PI3K/Akt; TRANSCRIPTION FACTOR POKEMON; REPRESSES TRANSCRIPTION; FBI-1; CANCER; PROGRESSION; ACTIVATION; DOMAIN; GENE;
D O I
10.3727/096504013X13657689383012
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Overexpression of Pokemon, which is an erythroid myeloid ontogenic factor protein, occurs in different cancers, including hepatocellular carcinoma (HCC). Pokemon is also reported to have an oncogenic activity in various human cancers. This study investigated the effect of Pokemon knockdown on the regulation of HCC growth. POK shRNA suppressed the expression of Pokemon protein in HepG2 cells compared to the negative control vector-transfected HCC cells. Pokemon knockdown also reduced HCC cell viability and enhanced cisplatin-induced apoptosis in HCC cells. AKT activation and the expression of various cell cycle-related genes were inhibited following Pokemon knockdown. These data demonstrate that Pokemon may play a role in HCC progression, suggesting that inhibition of Pokemon expression using Pokemon shRNA should be further evaluated as a novel target for the control of HCC.
引用
收藏
页码:377 / 381
页数:5
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