An intronic polyadenylation site in human and mouse CstF-77 genes suggests an evolutionarily conserved regulatory mechanism

被引：34

作者：

Pan, ZH ^{[1
]}

Zhang, HB ^{[1
]}

Hague, LK ^{[1
]}

Lee, JY ^{[1
]}

Lutz, CS ^{[1
]}

Tian, B ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Biochem & Mol Biol, Newark, NJ 07101 USA

来源：

GENE | 2006年 / 366卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

polyadenylation; gene regulation; CstF-77; SAGE;

D O I：

10.1016/j.gene.2005.09.024

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human CstF-77 is one of the three subunits of cleavage stimulation factor (CstF) that is essential for mRNA polyadenylation. Its Drosophila homologue, suppressor of forked [su(f)], contains in intronic poly(A) site, which can lead to a short transcript without a stop codon. By both bioinformatic searches and validation with molecular biology experiments, we found that human and mouse CstF-77 genes also contain an intronic poly(A) site, which can be utilized to produce short CstF-77 transcripts lacking sequences encoding domains that are involved in many of the CstF-77 functions. The genomic sequence surrounding the poly(A) site is highly conserved among all vertebrates, but is not present in nonvertebrate species. Using public Serial Analysis of Gene Expression (SAGE) data, we found that the intronic poly(A) site is utilized in a wide range of tissues. This finding indicates that vertebrates may employ a similar alternative polyadenylation mechanism to modulate CstF-77, highlighting the importance of the regulation of CstF-77 in various species. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：325 / 334

页数：10

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