Investigation of the Effect of Rice Wine on the Metabolites of the Main Components of Herbal Medicine in Rat Urine by Ultrahigh-Performance Liquid Chromatography-Quadrupole/Time-of-Flight Mass Spectrometry: A Case Study on Cornus officinalis

被引：5

作者：

Cao, Gang ^{[1
,2
,3
]}

Cai, Hao ^{[1
,3
]}

Yue, Xianke ^{[2
]}

Tu, Sicong ^{[4
]}

Cai, Baochang ^{[1
,2
,3
,5
]}

Xu, Zhiwei ^{[2
]}

机构：

[1] Nanjing Univ Chinese Med, Ctr Engn, State Minist Educ Standardizat Chinese Med Proc, Nanjing 210023, Jiangsu, Peoples R China

[2] Zhejiang Chinese Med Univ, Res Ctr TCM Proc Technol, Hangzhou 310053, Zhejiang, Peoples R China

[3] Nanjing Univ Chinese Med, Natl Class Key Discipline Sci Chinese Mat Med 1, Nanjing 210023, Jiangsu, Peoples R China

[4] Univ New S Wales, Fac Med, Sydney, NSW 2031, Australia

[5] Nanjing Haichang Chinese Med Grp Corp, Nanjing 210061, Jiangsu, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 2013卷

基金：

中国国家自然科学基金;

关键词：

PROCESSED FRUCTUS-CORNI; ION-TRAP; MORRONISIDE; CRUDE; IDENTIFICATION;

D O I：

10.1155/2013/306712

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Ultrahigh-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF/MS) was developed for rapid and sensitive analysis of the effect of rice wine on the metabolites of the main components of herbal medicine in rat urine. Using Cornus officinalis as a model of herbal medicine, the metabolite profiles of crude and processed (steaming the crude drug presteeped in rice wine) Cornus officinalis extracts in rat urine were investigated. The metabolites of Cornus officinalis were identified by using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. In this work, we identified the parent compounds and metabolites of crude and processed Cornus officinalis in rats. In total, three parent compounds and seventeen new metabolites of Cornus officinalis were found in rats. The contents of the parent compounds and metabolites in vivo varied significantly after intragastric (i.g.) administration of aqueous extracts of crude and processed Cornus officinalis. Data from this study suggests that UPLC-QTOF/MS could be used as a potential tool for uncovering the effects of excipients found in the metabolites of the main components of herbal medicine, in vivo, to predict and discover the processing mechanisms of herbal medicine.

引用

页数：10