Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.)

被引:17
作者
Bian, Miao [1 ,2 ,3 ,4 ]
Waters, Irene [2 ]
Broughton, Sue [2 ]
Zhang, Xiao-Qi [3 ]
Zhou, Meixue [4 ]
Lance, Reg [2 ]
Sun, Dongfa [1 ]
Li, Chengdao [2 ,3 ]
机构
[1] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
[2] Dept Agr & Food WA, S Perth, WA 6155, Australia
[3] Murdoch Univ, Western Australian State Agr Biotechnol Ctr, Murdoch, WA 6150, Australia
[4] Univ Tasmania, Tasmanian Inst Agr, Kings Meadows, Tas 7249, Australia
关键词
QTL; Aluminium tolerance; Gene-specific markers; Barley; CONTROLLING ALUMINUM TOLERANCE; MICROSATELLITE MARKERS; SEGREGATION DISTORTION; CROP PLANTS; SOIL-PH; IDENTIFICATION; DIVERSITY; ALP; MAP; INHERITANCE;
D O I
10.1007/s11032-013-9859-3
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Acid soil/aluminium toxicity is one of the major constraints on barley production around the world. Genetic improvement is the best solution and molecular-marker-assisted selection has proved to be an efficient tool for developing barley cultivars with acid soil/aluminium tolerance. In this study, barley variety Svanhals-introduced from CYMMIT (International Maize and Wheat Improvement Center)-was identified as acid soil/aluminium tolerant and the tolerance was mapped to chromosome 4H in 119 doubled haploid (DH) lines from a cross of Hamelin/Svanhals. The HvMATE gene, encoding an aluminium-activated citrate transporter, was selected as a candidate gene and gene-specific molecular markers were developed to detect acid soil/aluminium tolerance based on the polymerase chain reaction. Sequence analysis of the HvMATE gene identified a 21-bp indel (insertion-deletion) between the tolerant and sensitive cultivars. The new marker was further mapped to the QTL (quantitative trait loci) region on chromosome 4H for acid soil tolerance and accounted for 66.9 % of phenotypic variation in the DH population. Furthermore, the polymorphism was confirmed in other tolerant varieties which have been widely used as a source of acid soil tolerance in Australian barley breeding programs. The new gene-specific molecular marker provides an effective and simple molecular tool for selecting the acid soil tolerance gene from multiple tolerance sources.
引用
收藏
页码:155 / 164
页数:10
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