Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY581/591

被引：260
作者：
Pap, EHW
Drummen, GPC
Winter, VJ
Kooij, TWA
Rijken, P
Wirtz, KWA
Op den Kamp, JAF
Hage, WJ
Post, JA
机构：
[1] Univ Utrecht, Inst Biomembranes, Dept Biochem Lipids, NL-3584 CH Utrecht, Netherlands
[2] Univ Utrecht, Inst Biomembranes, Dept Mol Cell Biol, NL-3584 CH Utrecht, Netherlands
[3] Unilever Res Labs Vlaardingen, NL-3130 AC Vlaardingen, Netherlands
[4] Netherlands Inst Dev Biol, Hubrecht Lab, NL-3584 CT Utrecht, Netherlands
来源：
FEBS LETTERS | 1999年 / 453卷 / 03期
关键词：
BODIPY; lipid oxidation; oxygen radical; ratio-fluorescence microscopy; tocopherol;
D O I：
10.1016/S0014-5793(99)00696-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A ratio-fluorescence assay nas developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BOD-IPY581/591. Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membranes and is about twice as sensitive to oxidation as arachidonic acid. Using confocal microscopy, the cumene hydroperoxide-induced oxidation of C11-BODIPY581/591 was visualized at the sub-cellular level in rat-1 fibroblasts. Preloading of the cells with tocopherol retarded this oxidation, The data demonstrate that C11-BODIPY581/591 is a valuable tool to quantify lipid oxidation and anti-oxidant efficacy in single cells. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：278 / 282
页数：5
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