Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells

被引：7
|
作者：
Il'ina, I. V. [1 ]
Ovchinnikov, A. V. [1 ]
Chefonov, O. V. [1 ]
Sitnikov, D. S. [1 ]
Agranat, M. B. [1 ]
Mikaelyan, A. S. [2 ]
机构：
[1] Russian Acad Sci, Joint Inst High Temp, Moscow 125412, Russia
[2] Russian Acad Sci, NK Koltsov Dev Biol Inst, Moscow 119334, Russia
来源：
QUANTUM ELECTRONICS | 2013年 / 43卷 / 04期
关键词：
femtosecond laser pulses; laser microsurgery; optical transfection; cell membrane; optoporation; optoinjection; TRANSFECTION;
D O I：
10.1070/QE2013v043n04ABEH015115
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
IR femtosecond laser pulses were used for microsurgery of a cell membrane aimed at local and short-duration change in its permeability and injection of specified extracellular substances into the cells. The possibility of noncontact laser delivery of the propidium iodide fluorescent dye and the pEGFP plasmid, encoding the green fluorescent protein, into the cells with preservation of the cell viability was demonstrated.
引用
收藏
页码：365 / 369
页数：5
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