Antenatal betamethasone attenuates intrauterine infection-aggravated hyperoxia-induced lung injury in neonatal rats

被引：13

作者：

Yoo, Hye Soo ^{[1
]}

Chang, Yun Sil ^{[1
,2
]}

Kim, Jin Kyu ^{[3
]}

Ahn, So Yoon ^{[1
]}

Kim, Eun Sun ^{[1
]}

Sung, Dong Kyung ^{[2
]}

Jeon, Ga Won ^{[4
]}

Hwang, Jong Hee ^{[5
]}

Shim, Jae Won ^{[6
]}

Park, Won Soon ^{[1
,2
]}

机构：

[1] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Pediat, Seoul, South Korea

[2] Samsung Biomed Res Inst, Seoul, South Korea

[3] Chonbuk Natl Univ Hosp, Sch Med, Dept Pediat, Jeollabuk Do, South Korea

[4] Inje Univ, Coll Med, Pusan Paik Hosp, Dept Pediat, Pusan, South Korea

[5] Inje Univ, Coll Med, Ilsan Paik Hosp, Dept Pediat, Gyeonggi Do, South Korea

[6] Sungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Dept Pediat, Seoul, South Korea

来源：

PEDIATRIC RESEARCH | 2013年 / 73卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

ALVEOLAR COUNT METHOD; POSTNATAL LUNG; BRONCHOPULMONARY DYSPLASIA; INTRAAMNIOTIC ENDOTOXIN; PRETERM LAMBS; FETAL SHEEP; INFLAMMATION; MATURATION; GROWTH; CHORIOAMNIONITIS;

D O I：

10.1038/pr.2013.51

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

BACKGROUND: Intrauterine infection can exacerbate postnatal hyperoxic lung injury. We hypothesized that antenatal betamethasone treatment attenuates hyperoxic lung injury aggravated by intrauterine infection in neonatal rats. METHODS: Newborn Sprague-Dawley rats were divided into eight experimental groups according to (i) whether rats were exposed to normoxia (N) or hyperoxia (H, 85% oxygen) from postnatal day (P)1 to P14, (H) whether antenatal betamethasone (0.2 mg/dose) or vehicle was administered to pregnant rats at gestation days (E)19 and E20, and (Hi) whether intrauterine infection was induced or not antenatally. Intrauterine infection was induced by intracervical inoculation of Escherichia coli into pregnant rats on E19. We measured cytokine levels of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, and IL-1 beta in P1 rat lungs and performed morphometric analyses and assessed inflammatory responses in lung tissue and bronchoalveolar lavage (BAL) at P14 by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining and measurement of myeloperoxidase activity, collagen, and cytokine levels. RESULTS: Cytokine levels in P1 rat lungs were increased by intrauterine infection, and these increases were attenuated by antenatal betamethasone. Hyperoxic lung injuries, indicated by morphometric changes and an inflammatory response in the lung and BAL fluid, were aggravated by intrauterine infection at P14. This aggravation was significantly attenuated by antenatal betamethasone. CONCLUSION: Antenatal betamethasone attenuated aggravated hyperoxic lung injuries induced by intrauterine infection in neonatal rats via its anti-inflammatory actions.

引用

页码：726 / 733

页数：8

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