A cold water extract of Fucus vesiculosus inhibits lipopolysaccharide (LPS) induced pro-inflammatory responses in the porcine colon ex-vivo model

被引：18

作者：

Bahar, Bojlul ^{[1
]}

O'Doherty, John V. ^{[1
]}

Smyth, Thomas J. ^{[2
,3
]}

Ahmed, Albin M. ^{[4
]}

Sweeney, Torres ^{[4
]}

机构：

[1] Univ Coll Dublin, Sch Agr & Food Sci, Dublin 4, Ireland

[2] Teagasc Food Res Ctr, Food Biosci Dept, Dublin 15, Ireland

[3] Inst Technol Sligo, Dept Life Sci, Sligo, Ireland

[4] Univ Coll Dublin, Sch Vet Med, Dublin 4, Ireland

来源：

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES | 2016年 / 37卷

关键词：

Fucus; Functional food; Gene expression; Marine bioactives; Intestine; NF-KAPPA-B; IFN-GAMMA; TNF-ALPHA; SULFATED POLYSACCHARIDES; ASCOPHYLLUM-NODOSUM; MOLECULAR-WEIGHT; BROWN SEAWEEDS; ACTIVATION; LYSOZYME; CELLS;

D O I：

10.1016/j.ifset.2016.04.014

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The objectives of this experiment were to compare the in-vitro anti-inflammatory activity of crude extracts (cold water (CWE), hot water and 80% ethanol extract) of F. vesiculosus and to predict the key molecular targets of the extract with most anti-inflammatory activity in-vitro. In TNF-alpha. challenged Caco-2 cells, of the three crude extracts, the CWE exhibited maximum inhibition of IL-8 production. In the ex-vivo challenged porcine colonic tissue, CWE inhibited the expression (>2 fold) of inflammatory mediators (PTGS2, C5, LYZ), cytokines (IL17A, IL8), chemokines (CCL2, CXCL2, CXCL10, CXCL11), cell adhesion molecules (ICAM1, VCAM1), toll like receptors (TLR4, TLR7) and components of NF-kappa B (NFKB1, RELB), MAPIC (MAP3K8) and AP-1 (CJUN) pathways. The gene expression analysis suggest that the CWE does contain immunomodulatory bioactive compound/s that mediated through the interferon regulatory system 2, TNF-alpha inducing protein 3 and TNF-a receptor 2 and thus has potential application for human and animal health. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：229 / 236

页数：8

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