A Robust and Poisson Validated Quantitative 5′ Nuclease TaqMan® Real-Time PCR Assay Targeting fimA for the Rapid Detection of Salmonella spp. in Food

被引：10

作者：

Mann, E. ^{[1
]}

Hein, I. ^{[1
]}

Mester, P. ^{[2
]}

Stessl, B. ^{[1
]}

Rossmanith, P. ^{[1
,2
]}

Wagner, M. ^{[1
]}

Dzieciol, M. ^{[1
]}

机构：

[1] Univ Vet Med, Inst Milk Hyg Milk Technol & Food Sci IMMF, Dept Farm Anim & Vet Publ Hlth, A-1210 Vienna, Austria

[2] Univ Vet Med, Christian Doppler Lab Mol Food Analyt, Dept Farm Anim & Vet Publ Hlth, A-1210 Vienna, Austria

来源：

FOOD ANALYTICAL METHODS | 2013年 / 6卷 / 04期

关键词：

fimA; IAC; qPCR; Quantitative; Salmonella; LISTERIA-MONOCYTOGENES; GENE; AMPLIFICATION; TYPHIMURIUM; SEQUENCE; QUALITY; MEAT;

D O I：

10.1007/s12161-012-9534-z

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A newly designed TaqManA (R) probe and an internal amplification control were implemented in a conventional PCR system targeting the major fimbrial subunit encoding gene fimA. This assay has an inclusivity and exclusivity of 100 % (n = 126). The limit of detection (LOD) and the absolute quantification limit, both determined by advanced Poisson analyses, were three bacterial cell equivalents per quantitative real-time PCR reaction. The fimA assay achieved 100 % accuracy and performed very robustly, producing reproducible, reliable data for quantification of Salmonella spp., even at the LOD.

引用

页码：991 / 995

页数：5

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