A Label-Free Electrochemical Aptasensor for the Rapid Detection of Tetracycline Based on Ordered Mesoporous Carbon-Fe3O4

被引：5
作者：
Zhan, Xuejia [1 ,2 ,3 ]
Hu, Guangzhi [4 ,5 ]
Wagberg, Thomas [5 ]
Zhang, Dongwei [1 ,2 ,3 ]
Zhou, Pei [1 ,2 ,3 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
[2] Shanghai Jiao Tong Univ, Bor S Luh Food Safety Res Ctr, Shanghai 200240, Peoples R China
[3] Minist Agr, Key Lab Urban Agr South China, Guangzhou 510640, Guangdong, Peoples R China
[4] Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Lab Environm Sci & Technol, Urumqi 830011, Peoples R China
[5] Umea Univ, Dept Phys, S-90187 Umea, Sweden
来源：
AUSTRALIAN JOURNAL OF CHEMISTRY | 2018年 / 71卷 / 2-3期
基金：
中国国家自然科学基金;
关键词：
PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; MODIFIED ELECTRODE; APTAMER; BIOSENSOR; ANTIBIOTICS; NANOCOMPOSITE; NANOPARTICLES; NANOCRYSTALS; INHIBITION;
D O I：
10.1071/CH17503
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel aptasensor based on a tetracycline (TET) aptamer immobilized by physical adsorption on an ordered mesoporous carbon-Fe3O4 (OMC-Fe3O4)-modified screen-printed electrode surface was successfully fabricated. OMC-Fe3O4 was characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The modification procedure of the aptasensor was characterized by cyclic voltammetry. Interaction between the TET aptamer and target was determined by differential pulse voltammetry. Under optimal conditions, the proposed aptasensor exhibited good electrochemical sensitivity to TET in a concentration range of 5 nM to 10 mu M, with a detection limit of 0.8 nM (S/N = 3). This aptasensor exhibited satisfactory specificity, reproducibility, and stability.
引用
收藏
页码：170 / 176
页数：7
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