Biosynthesis of the Urease Metallocenter

被引：91
作者：
Farrugia, Mark A. [1 ]
Macomber, Lee [2 ]
Hausinger, Robert P. [1 ,2 ]
机构：
[1] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2013年 / 288卷 / 19期
基金：
美国国家卫生研究院;
关键词：
KLEBSIELLA-AEROGENES UREASE; HELICOBACTER-PYLORI UREASE; ALLELIC EXCHANGE MUTAGENESIS; ACCESSORY PROTEIN; CRYSTAL-STRUCTURE; NICKEL METALLOCENTER; BACILLUS-PASTEURII; GASTRIC PATHOGEN; BINDING PROTEIN; METAL-BINDING;
D O I：
10.1074/jbc.R112.446526
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Metalloenzymes often require elaborate metallocenter assembly systems to create functional active sites. The medically important dinuclear nickel enzyme urease provides an excellent model for studying metallocenter assembly. Nickel is inserted into the urease active site in a GTP-dependent process with the assistance of UreD/UreH, UreE, UreF, and UreG. These accessory proteins orchestrate apoprotein activation by delivering the appropriate metal, facilitating protein conformational changes, and possibly providing a requisite post-translational modification. The activation mechanism and roles of each accessory protein in urease maturation are the subject of ongoing studies, with the latest findings presented in this minireview.
引用
收藏
页码：13178 / 13185
页数：8
相关论文
共 85 条
[1]   Activation of the urease of Schizosaccharomyces pombe by the UreF accessory protein from soybean [J].
Bacanamwo, M ;
Witte, CP ;
Lubbers, MW ;
Polacco, JC .
MOLECULAR GENETICS AND GENOMICS, 2002, 268 (04) :525-534
[2]   Crystal Structure of the First Plant Urease from Jack Bean: 83 Years of Journey from Its First Crystal to Molecular Structure [J].
Balasubramanian, Anuradha ;
Ponnuraj, Karthe .
JOURNAL OF MOLECULAR BIOLOGY, 2010, 400 (03) :274-283
[3]   Crystallographic and X-ray absorption spectroscopic characterization of Helicobacter pylori UreE bound to Ni2+ and Zn2+ reveals a role for the disordered C-terminal arm in metal trafficking [J].
Banaszak, Katarzyna ;
Martin-Diaconescu, Vlad ;
Bellucci, Matteo ;
Zambelli, Barbara ;
Rypniewski, Wojciech ;
Maroney, Michael J. ;
Ciurli, Stefano .
BIOCHEMICAL JOURNAL, 2012, 441 :1017-1026
[4]   Allelic exchange mutagenesis of nixA in Helicobacter pylori results in reduced nickel transport and urease activity [J].
Bauerfeind, P ;
Garner, RM ;
Mobley, HLT .
INFECTION AND IMMUNITY, 1996, 64 (07) :2877-2880
[5]   Helicobacter pylori UreE, a urease accessory protein: specific Ni2+- and Zn2+-binding properties and interaction with its cognate UreG [J].
Bellucci, Matteo ;
Zambelli, Barbara ;
Musiani, Francesco ;
Turano, Paola ;
Ciurli, Stefano .
BIOCHEMICAL JOURNAL, 2009, 422 :91-100
[6]   A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii:: why urea hydrolysis casts two nickels [J].
Benini, S ;
Rypniewski, WR ;
Wilson, KS ;
Miletti, S ;
Ciurli, S ;
Mangani, S .
STRUCTURE, 1999, 7 (02) :205-216
[7]   Helicobacter pylori hydrogenase accessory protein HypA and urease accessory protein UreG compete with each other for UreE recognition [J].
Benoit, Stephane L. ;
McMurry, Jonathan L. ;
Hill, Stephanie A. ;
Maier, Robert J. .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2012, 1820 (10) :1519-1525
[8]   Klebsiella aerogenes UreF: Identification of the UreG Binding Site and Role in Enhancing the Fidelity of Urease Activation [J].
Boer, Jodi L. ;
Hausinger, Robert P. .
BIOCHEMISTRY, 2012, 51 (11) :2298-2308
[9]   Mutagenesis of Klebsiella aerogenes UreG To Probe Nickel Binding and Interactions with Other Urease-Related Proteins [J].
Boer, Jodi L. ;
Quiroz-Valenzuela, Soledad ;
Anderson, Kimberly L. ;
Hausinger, Robert P. .
BIOCHEMISTRY, 2010, 49 (28) :5859-5869
[10]   Novel genes affecting urease activity in Actinobacillus pleuropneumoniae [J].
Bossé, JT ;
Gilmour, HD ;
MacInnes, JI .
JOURNAL OF BACTERIOLOGY, 2001, 183 (04) :1242-1247
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