Inhibition of atopic dermatitis-like skin lesions by topical application of a novel ceramide derivative, K6PC-9p, in NC/Nga mice

被引：34

作者：

Kang, Jong Soon ^{[1
]}

Yoon, Won Kee ^{[1
]}

Youm, Jong-Kyung ^{[2
]}

Jeong, Se Kyoo ^{[2
]}

Park, Byeong Deog ^{[2
]}

Han, Mi Hwa ^{[1
]}

Lee, Hyunju ^{[1
]}

Moon, Eun-Yi ^{[3
]}

Han, Sang-Bae ^{[4
]}

Lee, Chang Woo ^{[1
]}

Lee, Kiho ^{[1
]}

Park, Song-Kyu ^{[1
]}

Yang, Kyu-Hwan ^{[5
]}

Kim, Hwan Mook ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Bioevaluat Ctr, Chungbuk, South Korea

[2] Neopharm Co Ltd, Taejon, South Korea

[3] Sejong Univ, Dept Biosci & Biotechnol, Seoul, South Korea

[4] Chungbuk Natl Univ, Coll Pharm, Cheongju, South Korea

[5] Kyungwon Univ, Gachon Bionano Res Inst, Kyunggido, South Korea

来源：

EXPERIMENTAL DERMATOLOGY | 2008年 / 17卷 / 11期

关键词：

atopic dermatitis; dust mite; K6PC-9p; NC/Nga; skin inflammation;

D O I：

10.1111/j.1600-0625.2008.00737.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Atopic dermatitis (AD) is a chronic inflammatory skin disease that commonly begins in childhood. K6PC-9p (N-(Ethyl dihydrogenphosphate)-2-hexyl-3-oxo-decanamide) is a synthetic ceramide derivative of PC-9S (N-Ethanol-2-mirystyl-3-oxo-staramide), which was known to be effective in atopic patients. In this study, we examined the effect of topical application of K6PC-9p on skin inflammation and AD-like skin lesions in mouse models. K6PC-9p dose-dependently inhibited phorbol ester-induced increase in ear thickness in BALB/c mice. Moreover, topical application of K6PC-9p suppressed dust mite extract-induced AD-like skin lesions in NC/Nga mice. Histopathological analysis revealed that both ear swelling and leucocyte infiltration were suppressed by K6PC-9p treatment. K6PC-9p also suppressed IL-4 and TNF-alpha expression in the ears and mast cell infiltration into the ears in NC/Nga mice. Further study demonstrated that K6PC-9p inhibited ConA-induced IL-4 secretion and LPS-induced macrophage activation. Taken together, our results showed that topical application of K6PC-9p exerts beneficial effects in animal model of skin inflammation and AD, suggesting that K6PC-9p might be a promising topical agent for the treatment of inflammatory skin diseases.

引用

页码：958 / 964

页数：7

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