Ghrelin is present in pancreatic α-cells of humans and rats and stimulates insulin secretion

被引：491
作者：
Date, Y
Nakazato, M [1 ]
Hashiguchi, S
Dezaki, K
Mondal, MS
Hosoda, H
Kojima, M
Kangawa, K
Arima, T
Matsuo, H
Yada, T
Matsukura, S
机构：
[1] Miyazaki Med Coll, Dept Internal Med 3, Miyazaki 8891692, Japan
[2] Natl Cardiovasc Ctr, Res Inst, Osaka, Japan
[3] Jichi Med Sch, Dept Physiol, Minami Kawachi, Tochigi 32904, Japan
[4] Kagoshima Univ, Fac Med, Dept Internal Med 2, Kagoshima 890, Japan
来源：
DIABETES | 2002年 / 51卷 / 01期
关键词：
D O I：
10.2337/diabetes.51.1.124
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Ghrelin, a novel growth hormone-releasing peptide isolated from human and rat stomach, is a 28-amino acid peptide with a posttranslational acylation modification that is indispensable for stimulating growth hormone secretion by increasing intracellular Ca2+ concentration. It also functions in the regulation of feeding behavior, energy metabolism, and gastric acid secretion and motility. Using two different antibodies against the NH2- and COOH-terminal regions of ghrelin, we studied its localization in human and rat pancreas by immunohistochemistry. Ghrelin-immunoreactive cells were identified at the periphery of pancreatic islets in both species. Ghrelin co-localized exclusively with glucagon in rat islets, indicating that it is produced in alpha-cells. We identified ghrelin and des-acyl ghrelin in the rat pancreas using reverse-phase high-performance liquid chromatography combined with two radioimmunoassays. We also detected mRNA encoding ghrelin and its receptor in the rat pancreatic islets. Ghrelin increased the cytosolic free Ca2+ concentration in beta-cells and stimulated insulin secretion when it was added to isolated rat pancreatic islets. These findings indicate that ghrelin may regulate islet function in an endocrine and/or paracrine manner.
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页码：124 / 129
页数：6
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