Prevalence and genotypes of Giardia duodenalis from dogs in Spain: possible zoonotic transmission and public health importance

被引：39

作者：

Dado, Diana ^{[1
,2
]}

Montoya, Ana ^{[1
]}

Alejandra Blanco, Maria ^{[2
,3
]}

Miro, Guadalupe ^{[1
]}

Maria Saugar, Jose ^{[2
]}

Bailo, Begona ^{[2
]}

Fuentes, Isabel ^{[2
]}

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Sanidad Anim, E-28040 Madrid, Spain

[2] ISCIII, Ctr Nacl Microbiol, Serv Parasitol, Madrid 28224, Spain

[3] Univ Los Andes, Fac Farm & Bioanal, Dept Microbiol & Parasitol, Merida, Venezuela

来源：

PARASITOLOGY RESEARCH | 2012年 / 111卷 / 06期

关键词：

MOLECULAR EPIDEMIOLOGY; PCR; CRYPTOSPORIDIUM; CYSTS; LAMBS; LOCUS; DNA;

D O I：

10.1007/s00436-012-3100-x

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The prevalence of Giardia duodenalis was determinate in faecal samples from dogs and cats in Madrid, Spain and molecular characterisation of isolates. A total of 604 and 144 faecal samples from dogs and cats, respectively, were analysed by routine coprological methods. The prevalence of G. duodenalis was 16.4 % (99/604) in dogs and 4.2 % (6/144) in cats. Sixty-four G. duodenalis isolates (63 from dogs and 1 from a cat) were characterised using glutamate dehydrogenase and beta-giardin genes by PCR-RFLP. The single cat sample showed a mixed infection by assemblages A + F. The assemblages found in the dog samples were A, B, C, D and E, both as single and as mixed infections. The zoonotic assemblages A and B were found in 56 (88.8 %) G. duodenalis-positive samples with 15.9 % of samples having assemblage A (10/63) and 73 % of samples with assemblage B (46/63), indicating high potential zoonotic risk and public health significance.

引用

页码：2419 / 2422

页数：4