Novel Mannan-PEG-PE Modified Bioadhesive PLGA Nanoparticles for Targeted Gene Delivery

被引：21

作者：

Wu, Guicun ^{[2
]}

Zhou, Fang ^{[1
]}

Ge, Linfu ^{[1
]}

Liu, Ximin ^{[1
]}

Kong, Fansheng ^{[1
]}

机构：

[1] PLA, Gen Hosp Jinan Command, Dept Hematol, Jinan 250031, Peoples R China

[2] Chinese Peoples Armed Forces, Shandong Prov Crops Hosp, Dept Hematol, Jinan, Peoples R China

来源：

JOURNAL OF NANOMATERIALS | 2012年 / 2012卷

关键词：

DRUG; NANOCARRIERS; LIPOSOMES; CHITOSAN; CELLS;

D O I：

10.1155/2012/981670

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Purpose. Biodegradable polymeric nanoparticles have been used frequently as gene delivery vehicles. The aim of this study is to modify bioadhesive PLGA nanoparticles with novel synthetic mannan-PEG-PE (MN-PEG-PE) to obtain active targeted gene delivery system. Methods. Mannan-PEG-PE ligands were synthesized and modified onto the NPs/pEGFP complexes. The modification rate was optimized, and the characteristics of the vehicle were evaluated. Then, the modified vectors were intravenous delivered to rats, and in vivo targeting behavior of MN-PEG-PE modified PLGA nanoparticles/pEGFP complexes (MN-PEG-PENPs/ pEGFP) in liver macrophages was investigated. Results. MN-PEG-PE-NPs/pEGFP displayed remarkably higher transfection efficiencies than nonmodified NPs/pEGFP both in vitro and in vivo. Conclusions. Mannan containing targeting ligands could significantly improve the transfection efficiency of the carriers. MN-PEG-PE modified vectors very useful in targeted gene delivery.

引用

页数：9

共 39 条

[1] Lipopolyamine-mediated single nanoparticle formation of calf thymus DNA analyzed by fluorescence correlation spectroscopy [J].

Adjimatera, Noppadon ;

Kral, Teresa ;

Hof, Martin ;

Blagbrough, Ian S. .

PHARMACEUTICAL RESEARCH, 2006, 23 (07) :1564-1573

[2] Recent advances on chitosan-based micro- and nanoparticles in drug delivery [J].

Agnihotri, SA ;

Mallikarjuna, NN ;

Aminabhavi, TM .

JOURNAL OF CONTROLLED RELEASE, 2004, 100 (01) :5-28

[3] PicoGreen quantitation of DNA: Effective evaluation of samples pre- or post-PCR [J].

Ahn, SJ ;

Costa, J ;

Emanuel, JR .

NUCLEIC ACIDS RESEARCH, 1996, 24 (13) :2623-2625

[4] Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences [J].

Atkinson, Helen ;

Chalmers, Ronald .

GENETICA, 2010, 138 (05) :485-498

[5] Mannose-binding plant lectins:: Different structural scaffolds for a common sugar-recognition process [J].

Barre, A ;

Bourne, Y ;

Van Damme, EJM ;

Peumans, WJ ;

Rougé, P .

BIOCHIMIE, 2001, 83 (07) :645-651

[6] Non-viral and viral vectors for gene therapy [J].

Boulaiz, H ;

Marchal, JA ;

Prados, J ;

Melguizo, C ;

Aránega, A .

CELLULAR AND MOLECULAR BIOLOGY, 2005, 51 (01) :3-22

[7] Cyclodextrin-based siRNA delivery nanocarriers: a state-of-the-art review [J].

Chaturvedi, Kiran ;

Ganguly, Kuntal ;

Kulkarni, Anandrao R. ;

Kulkarni, Venkatarao H. ;

Nadagouda, Mallikarjuna N. ;

Rudzinski, Walter E. ;

Aminabhavi, Tejraj M. .

EXPERT OPINION ON DRUG DELIVERY, 2011, 8 (11) :1455-1468

[8] A multifunctional nano device as non-viral vector for gene delivery: In vitro characteristics and transfection [J].

Gao, Yu ;

Gu, Wangwen ;

Chen, Lingli ;

Xu, Zhenghong ;

Li, Yaping .

JOURNAL OF CONTROLLED RELEASE, 2007, 118 (03) :381-388

[9] A novel isolation method for macrophage-like cells from mixed primary cultures of adult rat liver cells [J].

Kitani, Hiroshi ;

Takenouchi, Takato ;

Sato, Mitsuru ;

Yoshioka, Miyako ;

Yamanaka, Noriko .

JOURNAL OF IMMUNOLOGICAL METHODS, 2010, 360 (1-2) :47-55

[10] High Molecular Weight Polyglycerol-Based Multivalent Mannose Conjugates [J].

Kizhakkedathu, Jayachandran N. ;

Creagh, A. Louise ;

Shenoi, Rajesh A. ;

Rossi, Nicholas A. A. ;

Brooks, Donald E. ;

Chan, Timmy ;

Lam, Jonathan ;

Dandepally, Srinivasa R. ;

Haynes, Charles A. .

BIOMACROMOLECULES, 2010, 11 (10) :2567-2575

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