LC-MS-MS Method for the Determination of Protoveratrine A in Rat Plasma

被引：4
|
作者：
Li, Hai-Yun [1 ]
Xu, Wen [1 ]
Li, Hui-Liang [1 ]
Zhang, Wei-Dong [1 ,2 ]
Hu, Li-Wei [1 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Dept Nat Med Chem, Shanghai 200433, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200030, Peoples R China
来源：
CHROMATOGRAPHIA | 2009年 / 69卷 / 5-6期
关键词：
Column liquid chromatography-mass spectrometry; Tandem mass spectrometry; Protoveratrine A in rat plasma; STEROIDAL ALKALOIDS; CYCLOPAMINE; DERIVATIVES; INHIBITION;
D O I：
10.1365/s10337-008-0936-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The present experiments were designed for the development of a liquid chromatographic coupled with tandem mass spectrometric method for the quantitation of trace amount of protoveratrine A in rat plasma. A liquid-liquid extraction protocol was involved in sample preparation. Quantitation was achieved using multiple-reaction-monitoring (MRM) mode. The method was validated with the concentration range 0.02-40 ngmL(-1) in plasma. Intra-/inter-day accuracy and precision as well as the LOD and the lower limit of quantitation were acceptable. The method was further applied to analyze protoveratrine A in rat plasma samples.
引用
收藏
页码：523 / 529
页数：7
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