Characterization of a novel primary culture system of adult zebrafish brainstem cells

被引：9

作者：

Tapanes-Castillo, Alexis ^{[1
]}

Shabazz, Francelethia S. ^{[1
]}

Mboge, Mam Y. ^{[1
]}

Vajn, Katarina ^{[2
]}

Oudega, Martin ^{[2
,3
,4
]}

Plunkett, Jeffery A. ^{[1
]}

机构：

[1] St Thomas Univ, Sch Sci Technol & Engn Management, Miami Gardens, FL 33054 USA

[2] Univ Pittsburgh, Sch Med, Dept Phys Med & Rehabil, Pittsburgh, PA USA

[3] Univ Pittsburgh, Sch Med, Dept Neurobiol, Pittsburgh, PA USA

[4] Univ Pittsburgh, Sch Med, Dept Bioengn, Pittsburgh, PA USA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2014年 / 223卷

关键词：

Danio rerio; CNS; Neuron; Glia; Stem cell; Brainstem; Regeneration; FIBRILLARY ACIDIC PROTEIN; RADIAL GLIAL-CELLS; AXONAL REGENERATION; MOTOR-NEURONS; EXPRESSION; INHIBITION; GROWTH; NESTIN; LOCALIZATION; NEUROGENESIS;

D O I：

10.1016/j.jneumeth.2013.11.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adult zebrafish (Danio rerio) have a remarkable ability to restore function after an injury to the brain or spinal cord. The molecular and cellular mechanisms underlying this phenomenon are not fully understood. To enable investigation of these mechanisms we have developed an in vitro model system from the adult zebrafish brainstem, which can be maintained under serum-containing and serum-free conditions. While cultures are predominantly neuronal, they also contain glia and stem progenitor cells. Various stages of cellular differentiation are observed among both neuronal and non-neuronal populations. Quantitative morphological results revealed typical cellular growth over a two-week period. We argue that our novel brainstem culture model offers a powerful tool for the studies of axonal growth, neurogenesis, and regeneration in the adult zebrafish central nervous system. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：11 / 19

页数：9

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